Document Detail


A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry.
MedLine Citation:
PMID:  19002869     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.
Authors:
Laurent Foiry; Jérôme Mégret; Claudine Junien; Geneviève Gourdon
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Publication Detail:
Type:  Journal Article     Date:  2006-12-14
Journal Detail:
Title:  Cytotechnology     Volume:  52     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-02-19    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-12     Citation Subset:  -    
Affiliation:
Inserm U781, Clinique M. Lamy, Hôpital Necker Enfants Malades, 149 rue de Sèvres, 75015, Paris, France.
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