Document Detail


A simple and fast electrophoretic method for elution of nucleic acids from gels.
MedLine Citation:
PMID:  530273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.
Authors:
Y Chouikh; M Volovitch; P Yot
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular biology reports     Volume:  5     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-04-23     Completed Date:  1980-04-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  237-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA / isolation & purification*
Electrophoresis / methods*
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
RNA / isolation & purification*
Chemical
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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