Document Detail

A simple and fast electrophoretic method for elution of nucleic acids from gels.
MedLine Citation:
PMID:  530273     Owner:  NLM     Status:  MEDLINE    
A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.
Y Chouikh; M Volovitch; P Yot
Related Documents :
508803 - Glycoproteins from experimental granulation tissue and their effects on collagen synthe...
16959313 - Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid g...
17943203 - Effects of oligodna template length and sequence on binary self-assembly of a nucleotid...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular biology reports     Volume:  5     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-04-23     Completed Date:  1980-04-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  237-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA / isolation & purification*
Electrophoresis / methods*
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
RNA / isolation & purification*
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Tissue-specific ribosomal protein composition.
Next Document:  Histone: oxidation by peroxidase alters its interaction with DNA.