Document Detail


A simple and efficient method for purifying and quantifying schizonts from Theileria parva-infected cells.
MedLine Citation:
PMID:  1924254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved method for the purification of Theileria parva schizonts from infected bovine cells is described. The technique is simpler and more rapid than previously described methods and gives rise to greater yields of schizonts with negligible contamination by host-cell components. In addition, a fluorescent staining technique was developed whereby live schizonts purified from infected cells can be enumerated and sorted using the flow cytometer. An assessment of the quality of schizonts prepared according to our method as a source of RNA for the construction of parasite cDNA libraries suggests that RNA derived from these preparations is free of host nucleic acids.
Authors:
B M Goddeeris; S Dunlap; E A Innes; D J McKeever
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Parasitology research     Volume:  77     ISSN:  0932-0113     ISO Abbreviation:  Parasitol. Res.     Publication Date:  1991  
Date Detail:
Created Date:  1991-11-21     Completed Date:  1991-11-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  482-4     Citation Subset:  IM    
Affiliation:
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Cattle
Cell Line
Flow Cytometry
RNA, Protozoan / analysis*,  isolation & purification
Theileria parva / genetics,  growth & development,  isolation & purification*
Chemical
Reg. No./Substance:
0/RNA, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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