Document Detail

A simple dual pressure-ejection system and calibration method for brief local applications of drugs and modified salines.
MedLine Citation:
PMID:  8544493     Owner:  NLM     Status:  MEDLINE    
We report a minimal method for dual pressure ejection through a single micropipette, which uses standard theta-type glass tubing and a connection through thinned polythene catheters without any sealing. We also describe a simple calibration method using a standard electrometer. The linearity of the ejected volume with respect to pulse duration and to applied pressure was maintained, as measured by the resistivity of ultrapure water after ejection of a saline solution. The method was used to analyse the inhibitory effect of glutamate on crayfish motoneurones with local and reversible applications of low-chloride saline and picrotoxin.
A R Marchand; E Pearlstein
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  60     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1996-02-14     Completed Date:  1996-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
CNRS, Laboratoire Neurobiologie et Mouvements, Marseille, France.
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MeSH Terms
Astacoidea / physiology
Chlorides / pharmacology
Glutamic Acid / administration & dosage,  pharmacology
Microinjections / instrumentation*
Motor Neurons / drug effects,  physiology
Sodium Chloride
Virulence Factors, Bordetella / administration & dosage,  pharmacology
Reg. No./Substance:
0/Chlorides; 0/Virulence Factors, Bordetella; 56-86-0/Glutamic Acid; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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