| A simple dual pressure-ejection system and calibration method for brief local applications of drugs and modified salines. | |
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MedLine Citation:
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PMID: 8544493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a minimal method for dual pressure ejection through a single micropipette, which uses standard theta-type glass tubing and a connection through thinned polythene catheters without any sealing. We also describe a simple calibration method using a standard electrometer. The linearity of the ejected volume with respect to pulse duration and to applied pressure was maintained, as measured by the resistivity of ultrapure water after ejection of a saline solution. The method was used to analyse the inhibitory effect of glutamate on crayfish motoneurones with local and reversible applications of low-chloride saline and picrotoxin. |
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Authors:
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A R Marchand; E Pearlstein |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 60 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1996-02-14 Completed Date: 1996-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 99-105 Citation Subset: IM |
Affiliation:
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CNRS, Laboratoire Neurobiologie et Mouvements, Marseille, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astacoidea / physiology Calibration Chlorides / pharmacology Glutamic Acid / administration & dosage, pharmacology Microinjections / instrumentation* Motor Neurons / drug effects, physiology Pressure Sodium Chloride Virulence Factors, Bordetella / administration & dosage, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Virulence Factors, Bordetella; 56-86-0/Glutamic Acid; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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