| A simple colony-blot method for identification of Listeria in food samples. | |
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MedLine Citation:
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PMID: 8577309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for identification of Listeria in food samples was developed. It consisted of cultivating of suspected specimen on standard agar medium, direct absorption of grown colonies onto nitrocellulose membrane and processing of the latter with rabbit serum raised against purified cell wall protein Lm79/39 of L. monocytogenes. Analysis using anti-rabbit peroxidase conjugate and 4-chloro-1-naphthol and H2O2 solutions allowed direct detection of Listeria colonies which remained readily available for subsequent isolation. |
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Authors:
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Y F Belyi; N A Varfolomeeva; I S Tartakovskii |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical microbiology and immunology Volume: 184 ISSN: 0300-8584 ISO Abbreviation: Med. Microbiol. Immunol. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1996-03-13 Completed Date: 1996-03-13 Revised Date: 2007-02-21 |
Medline Journal Info:
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Nlm Unique ID: 0314524 Medline TA: Med Microbiol Immunol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 105-8 Citation Subset: IM |
Affiliation:
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N.F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. bel@gamem.msk.su |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial Antibody Specificity Antigens, Bacterial / immunology Bacterial Proteins / immunology Cell Wall / immunology Colony Count, Microbial / methods* Immunoblotting / methods* Listeria / immunology, isolation & purification* Milk / microbiology* Rabbits Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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