Document Detail


A simple colony-blot method for identification of Listeria in food samples.
MedLine Citation:
PMID:  8577309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for identification of Listeria in food samples was developed. It consisted of cultivating of suspected specimen on standard agar medium, direct absorption of grown colonies onto nitrocellulose membrane and processing of the latter with rabbit serum raised against purified cell wall protein Lm79/39 of L. monocytogenes. Analysis using anti-rabbit peroxidase conjugate and 4-chloro-1-naphthol and H2O2 solutions allowed direct detection of Listeria colonies which remained readily available for subsequent isolation.
Authors:
Y F Belyi; N A Varfolomeeva; I S Tartakovskii
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  184     ISSN:  0300-8584     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-03-13     Completed Date:  1996-03-13     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  105-8     Citation Subset:  IM    
Affiliation:
N.F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. bel@gamem.msk.su
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial
Antibody Specificity
Antigens, Bacterial / immunology
Bacterial Proteins / immunology
Cell Wall / immunology
Colony Count, Microbial / methods*
Immunoblotting / methods*
Listeria / immunology,  isolation & purification*
Milk / microbiology*
Rabbits
Species Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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