| A simple backscattering microscope for fast tracking of biological molecules. | |
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MedLine Citation:
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PMID: 21133475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent developments in techniques for observing single molecules under light microscopes have helped reveal the mechanisms by which molecular machines work. A wide range of markers can be used to detect molecules, from single fluorophores to micron sized markers, depending on the research interest. Here, we present a new and simple objective-type backscattering microscope to track gold nanoparticles with nanometer and microsecond resolution. The total noise of our system in a 55 kHz bandwidth is ~0.6 nm per axis, sufficient to measure molecular movement. We found our backscattering microscopy to be useful not only for in vitro but also for in vivo experiments because of lower background scattering from cells than in conventional dark-field microscopy. We demonstrate the application of this technique to measuring the motion of a biological rotary molecular motor, the bacterial flagellar motor, in live Escherichia coli cells. |
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Authors:
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Yoshiyuki Sowa; Bradley C Steel; Richard M Berry |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Review of scientific instruments Volume: 81 ISSN: 1089-7623 ISO Abbreviation: Rev Sci Instrum Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-12-07 Completed Date: 2011-04-15 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0405571 Medline TA: Rev Sci Instrum Country: United States |
Other Details:
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Languages: eng Pagination: 113704 Citation Subset: IM |
Affiliation:
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Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 184-8584, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Darkness Escherichia coli / cytology Flagella / metabolism Gold / chemistry Lasers Metal Nanoparticles / chemistry Microscopy / methods* Particle Size Scattering, Radiation* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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BB/E00458X/1//Biotechnology and Biological Sciences Research Council; R01GM066223/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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