Document Detail


A simple assay for optimizing yeast-mammalian cell fusion conditions.
MedLine Citation:
PMID:  8970165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyethylene glycol (PEG)-induced cell fusion can be a useful method for the transfer of yeast artificial chromosomes (YACs) from yeast spheroplasts to mammalian cells in culture, although success varies between recipient cell types. Experiments aimed at determining optimum fusion conditions can also be very time-consuming. To minimize this difficulty, a reporter plasmid has been constructed that allows yeast-mammalian cell fusion rates to be determined within 3 d. The speed and sensitivity of the assay should allow a more systematic evaluation of cell lines for their capacity to fuse with yeast, and for rapid optimization of fusion parameters.
Authors:
D Markie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biotechnology     Volume:  6     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-03-12     Completed Date:  1997-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Affiliation:
Paediatric Research Unit, UMDS, Guy's Hospital, London. david.markie@stonebow.otago.ac.nz
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Fusion*
Chromosomes, Artificial, Yeast
Cricetinae
Genes, Reporter
Genetic Techniques*
Plasmids
Saccharomyces cerevisiae / cytology*,  genetics
Selection, Genetic
Sensitivity and Specificity
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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