Document Detail

A simple assay for optimizing yeast-mammalian cell fusion conditions.
MedLine Citation:
PMID:  8970165     Owner:  NLM     Status:  MEDLINE    
Polyethylene glycol (PEG)-induced cell fusion can be a useful method for the transfer of yeast artificial chromosomes (YACs) from yeast spheroplasts to mammalian cells in culture, although success varies between recipient cell types. Experiments aimed at determining optimum fusion conditions can also be very time-consuming. To minimize this difficulty, a reporter plasmid has been constructed that allows yeast-mammalian cell fusion rates to be determined within 3 d. The speed and sensitivity of the assay should allow a more systematic evaluation of cell lines for their capacity to fuse with yeast, and for rapid optimization of fusion parameters.
D Markie
Related Documents :
3032215 - Cell fusion by nasopharyngeal carcinoma-derived epstein-barr virus.
20562485 - Gaas nanowire/poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) hybrid solar cells.
10842195 - Genetic complementation to non-tumorigenicity in cervical-carcinoma cells correlates wi...
8477745 - Vigilin is a cytoplasmic protein. a study on its expression in primary cells and in est...
9549625 - Biocompatibility analysis of different biomaterials in human bone marrow cell cultures.
7811945 - Multiple site optical recording of transmembrane voltage (msortv) in patterned growth h...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biotechnology     Volume:  6     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-03-12     Completed Date:  1997-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Paediatric Research Unit, UMDS, Guy's Hospital, London.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
CHO Cells
Cell Fusion*
Chromosomes, Artificial, Yeast
Genes, Reporter
Genetic Techniques*
Saccharomyces cerevisiae / cytology*,  genetics
Selection, Genetic
Sensitivity and Specificity
beta-Galactosidase / metabolism
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structure of pp32, an acidic nuclear protein which inhibits oncogene-induced formation of transforme...
Next Document:  Tat-mediated protein delivery can facilitate MHC class I presentation of antigens.