Document Detail

A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria.
MedLine Citation:
PMID:  10747246     Owner:  NLM     Status:  MEDLINE    
A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.
J McKerrow; S Vagg; T McKinney; E M Seviour; A M Maszenan; P Brooks; R J Seviour
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  30     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-12     Completed Date:  2000-05-12     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  178-82     Citation Subset:  IM    
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
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MeSH Terms
Cell Wall / chemistry
Chromatography, High Pressure Liquid / methods
Diaminopimelic Acid / analysis*
Gram-Positive Bacteria / chemistry*
Reg. No./Substance:
583-93-7/Diaminopimelic Acid

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