| A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. | |
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MedLine Citation:
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PMID: 10747246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required. |
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Authors:
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J McKerrow; S Vagg; T McKinney; E M Seviour; A M Maszenan; P Brooks; R J Seviour |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Letters in applied microbiology Volume: 30 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-05-12 Completed Date: 2000-05-12 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 178-82 Citation Subset: IM |
Affiliation:
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Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Wall
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chemistry Chromatography, High Pressure Liquid / methods Diaminopimelic Acid / analysis* Gram-Positive Bacteria / chemistry* |
| Chemical | |
Reg. No./Substance:
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583-93-7/Diaminopimelic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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