| A silkworm larvae plasma test for detecting peptidoglycan in cerebrospinal fluid is useful for the diagnosis of bacterial meningitis. | |
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MedLine Citation:
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PMID: 14605436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The silkworm larvae plasma (SLP) test has been established based on a cascade reaction triggered by either peptidoglycan or (1, 3)-beta-D-glucan to produce melanin. We applied this test to the diagnosis of bacterial meningitis. Cerebrospinal fluid (CSF) obtained from patients with bacterial meningitis due to gram-positive bacteria, gram-negative bacteria, or fungi, showed positive reactions to the test. In contrast, CSF from patients with viral meningitis or noninfectious illnesses gave negative reactions. Therefore, this test seems to be useful for diagnosis of bacterial and fungal meningitis. When this test was used together with two types of limulus tests, an endotoxin-specific test, and a conventional test, meningitis was further characterized as gram-positive, gram-negative or fungal meningitis. The SLP test requires a computerized instrument for quantitative colorimetric measurement. A qualitative alternative of this test also can be accomplished by visually observing the darkening color. Thus, this method can be applied for simple and rapid diagnosis of meningitis. |
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Authors:
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Katsuya Inada; Kiyomi Takahashi; Sadato Ichinohe; Hidetoshi Suda; Masakazu Tsuchiya; Junkichi Takahashi; Shuji Matsuura; Takeshi Kasai; Masao Yoshida; Shigeatsu Endo; Shigehiro Sato |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology and immunology Volume: 47 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-11-07 Completed Date: 2004-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: Japan |
Other Details:
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Languages: eng Pagination: 701-7 Citation Subset: IM |
Affiliation:
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Department of Microbiology, School of Medicine, Iwate Medical University, Iwate 020-8505, Japan. kaind@mb.neweb.ne.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Animals Biological Assay Bombyx* / growth & development Cerebrospinal Fluid / chemistry* Child Child, Preschool Female Gram-Negative Bacteria / chemistry* Gram-Positive Bacteria / chemistry* Hemolymph Humans Infant Infant, Newborn Larva Male Meningitis, Bacterial / diagnosis, microbiology* Peptidoglycan / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Peptidoglycan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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