Document Detail

The significance of differences in fatty acid metabolism between obese and non-obese patients with non-alcoholic fatty liver disease.
MedLine Citation:
PMID:  18949388     Owner:  NLM     Status:  MEDLINE    
Non-alcoholic fatty liver disease (NAFLD) is considered to be associated with metabolic syndrome; however, a number of NAFLD patients are not obese. To explore any differences in lipid metabolism between obese and non-obese patients, we determined the expression of fatty acid metabolism-related genes. Expression levels of target genes were quantified by real-time PCR using liver biopsy samples from NAFLD patients and normal controls. Serum adipocytokine levels were also determined. The expression of genes related to fatty acid synthesis and uptake was generally up-regulated in NAFLD patients; however, no significant difference was seen between obese and non-obese groups. Most of the genes tested related to fatty acid and reactive oxygen species (ROS) elimination, were overexpressed in NAFLD and the levels were significantly higher in non-obese patients. As an exception, peroxisome proliferator-activated receptor alpha expression was suppressed in NAFLD and the levels were lower in the obese group. Triglyceride synthesis-related genes were up-regulated and lipolytic enzymes were decreased in NAFLD, but there was no significant difference between the obese and non-obese groups. In NAFLD, increased de novo synthesis and uptake of fatty acids led to further hepatocyte accumulation of fatty acids. The up-regulation of fatty acid oxidation and the antioxidant pathway and the suppression of lipolysis seemed to be involved in this process. Expression of genes related to fatty acid oxidation and ROS elimination were higher in the non-obese group than in the obese group, which contributes to the trend of more severe liver injury, insulin resistance and steatosis in obese patients.
Makoto Nakamuta; Motoyuki Kohjima; Nobito Higuchi; Masaki Kato; Kazuhiro Kotoh; Tsuyoshi Yoshimoto; Masayoshi Yada; Ryoko Yada; Ryosuke Takemoto; Kunitaka Fukuizumi; Naohiko Harada; Akinobu Taketomi; Yoshihiko Maehara; Manabu Nakashima; Munechika Enjoji
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  22     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-24     Completed Date:  2009-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  663-7     Citation Subset:  IM    
Department of Gastroenterology, Kyushu Medical Center, National Hospital Organization, Fukuoka, Japan.
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MeSH Terms
Adipokines / biosynthesis,  genetics
Fatty Acids / genetics,  metabolism*
Fatty Liver / genetics,  metabolism*,  pathology
Gene Expression Regulation / genetics
Insulin Resistance / genetics
Metabolic Syndrome X / genetics,  metabolism*,  pathology
Obesity / genetics,  metabolism*,  pathology
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Adipokines; 0/Fatty Acids; 0/Reactive Oxygen Species

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