Document Detail


The significance of beta-APP immunoreactivity in forensic practice.
MedLine Citation:
PMID:  15885067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neuropathologist involved in forensic work is not uncommonly confronted with a case in which there is no or only a limited history or, if available, the information is uncertain or is often conflicting. In recent years the immunohistochemical stain beta-amyloid precursor protein (beta-APP) has been used to assess the extent of axonal injury in a variety of pathological processes but in forensic practice is of greatest utility in the assessment of traumatic brain injury. Diffuse traumatic axonal injury (TAI) in humans has been demonstrated by beta-APP immunoreactivity in patients surviving at least 2 h after head injury. However, many of these patients also have an associated ischaemic injury, either focal or diffuse, which may make the interpretation of beta-APP immunoreactivity difficult. The present study was designed to evaluate if the published descriptions of the different morphological patterns and distributions of beta-APP immunoreactive axons could be used to microscopically distinguish axonal injury attributed to trauma from other causes. To test this hypothesis a total of 73 cases were reviewed. The cases were selected from six different groups based on clinical information. Immunostained sections from each case were assessed 'blind' to the clinical history, and the microscopic pattern and distribution of beta-APP positive axons were recorded. Haematoxylin and eosin (H+E) stained sections were then reviewed for each case and a final pathological diagnosis was recorded and compared to the clinical history. 62/73 (85%) cases were correctly correlated with the clinical history and in particular 14/17 (82%) cases of TAI were correctly identified. These findings indicate that the published microscopic patterns of the distribution of beta-APP positive axons in TAI and in diffuse ischaemic injury can be used, in conjunction with microscopy of H+E stained sections to determine the cause of axonal pathology in most cases.
Authors:
R R Reichard; C Smith; D I Graham
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Neuropathology and applied neurobiology     Volume:  31     ISSN:  0305-1846     ISO Abbreviation:  Neuropathol. Appl. Neurobiol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-11     Completed Date:  2005-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7609829     Medline TA:  Neuropathol Appl Neurobiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  304-13     Citation Subset:  IM    
Affiliation:
Academic Unit of Neuropathology, University of Glasgow, Institute of Neurological Sciences, Southern General Hospital, Glasgow G51 4TF, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Amyloid beta-Protein Precursor / metabolism*
Brain Injuries / diagnosis*,  etiology,  metabolism
Brain Ischemia / diagnosis,  etiology,  pathology
Carbon Monoxide Poisoning / complications
Child
Diagnosis, Differential
Female
Forensic Pathology*
Heart Arrest / complications
Humans
Hypoglycemia / complications
Immunohistochemistry
Male
Middle Aged
Reproducibility of Results
Sensitivity and Specificity
Status Epilepticus / complications
Chemical
Reg. No./Substance:
0/Amyloid beta-Protein Precursor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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