Document Detail

The significance of MMP-1 and MMP-2 in peritoneal disseminated metastasis of gastric cancer.
MedLine Citation:
PMID:  10930227     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to clarify the role of the matrix metalloproteinases (MMPs), collagenase (MMP-1), and gelatinase A (MMP-2), both of which are known to be involved in the development of gastric cancer, in peritoneal dissemination. The concentrations of MMP-1 and MMP-2 in the supernatant of mixed culture simulated peritoneal dissemination were measured in vitro with mesothelial cells and cancer cells using an enzyme-linked immunosorbent assay. The concentration of MMP-1 increased significantly after the contact culture was mixed with these two cells, in comparison with the non-contact mixed culture or the mesothelial cell culture alone. These results demonstrate that the production of MMP-1 derived from mesothelial cells was increased by contact with cancer cells. To clarify the effect of MMP-1 and MMP-2 on gastric cancer invasion, an invasion assay using matrigel was performed. After treatment with anti-MMP-1 monoclonal antibody (mAb) or anti-MMP-2 mAb, the number of matrigel-penetrating cancer cells was significantly reduced, indicating that MMP-1 and MMP-2 derived from mesothelial cells had a strong reaction to gastric cancer invasion. In conclusion, as MMP-1 showed a paracrine-like action responding to stimulus from cancer cells, it seemed to play an important role in the progression of peritoneal dissemination.
K Mizutani; K Kofuji; K Shirouzu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Surgery today     Volume:  30     ISSN:  0941-1291     ISO Abbreviation:  Surg. Today     Publication Date:  2000  
Date Detail:
Created Date:  2000-11-13     Completed Date:  2000-12-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204360     Medline TA:  Surg Today     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  614-21     Citation Subset:  IM    
Department of Surgery, Kurume University, School of Medicine, Fukuoka, Japan.
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MeSH Terms
Cell Culture Techniques / methods
Matrix Metalloproteinase 1 / analysis,  pharmacology*
Matrix Metalloproteinase 2 / analysis,  pharmacology*
Neoplasm Invasiveness
Peritoneal Neoplasms / pathology,  secondary*
Stomach Neoplasms / pathology*
Tumor Cells, Cultured / physiology
Reg. No./Substance:
EC Metalloproteinase 2; EC Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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