| The signal transduction function for oxidative phosphorylation is at least second order in ADP. | |
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MedLine Citation:
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PMID: 8910406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To maintain ATP constant in the cell, mitochondria must sense cellular ATP utilization and transduce this demand to F0-F1-ATPase. In spite of a considerable research effort over the past three decades, no combination of signal(s) and kinetic function has emerged with the power to explain ATP homeostasis in all mammalian cells. We studied this signal transduction problem in intact human muscle using 31P NMR spectroscopy. We find that the apparent kinetic order of the transduction function of the signal cytosolic ADP concentration ([ADP]) is at least second order and not first order as has been assumed. We show that amplified mitochondrial sensitivity to cytosolic [ADP] harmonizes with in vitro kinetics of [ADP] stimulation of respiration and explains ATP homeostasis also in mouse liver and canine heart. This result may well be generalizable to all mammalian cells. |
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Authors:
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J A Jeneson; R W Wiseman; H V Westerhoff; M J Kushmerick |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 271 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1996-12-30 Completed Date: 1996-12-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27995-8 Citation Subset: IM |
Affiliation:
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NMR Research Laboratory, Department of Radiology, University of Washington School of Medicine, Seattle, Washington 98195, USA. utrecht@u.washington.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism* Animals Cytosol / metabolism Dogs Energy Metabolism Homeostasis Humans Kinetics Magnetic Resonance Spectroscopy Mice Mitochondria, Muscle / metabolism* Muscle, Skeletal / metabolism Oxidative Phosphorylation* Oxygen Consumption Proton-Translocating ATPases / metabolism Signal Transduction* |
| Grant Support | |
ID/Acronym/Agency:
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AR36281/AR/NIAMS NIH HHS; AR41793/AR/NIAMS NIH HHS; AR41928/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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58-64-0/Adenosine Diphosphate; EC 3.6.3.14/Proton-Translocating ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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