Document Detail


The signal transduction function for oxidative phosphorylation is at least second order in ADP.
MedLine Citation:
PMID:  8910406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To maintain ATP constant in the cell, mitochondria must sense cellular ATP utilization and transduce this demand to F0-F1-ATPase. In spite of a considerable research effort over the past three decades, no combination of signal(s) and kinetic function has emerged with the power to explain ATP homeostasis in all mammalian cells. We studied this signal transduction problem in intact human muscle using 31P NMR spectroscopy. We find that the apparent kinetic order of the transduction function of the signal cytosolic ADP concentration ([ADP]) is at least second order and not first order as has been assumed. We show that amplified mitochondrial sensitivity to cytosolic [ADP] harmonizes with in vitro kinetics of [ADP] stimulation of respiration and explains ATP homeostasis also in mouse liver and canine heart. This result may well be generalizable to all mammalian cells.
Authors:
J A Jeneson; R W Wiseman; H V Westerhoff; M J Kushmerick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-30     Completed Date:  1996-12-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27995-8     Citation Subset:  IM    
Affiliation:
NMR Research Laboratory, Department of Radiology, University of Washington School of Medicine, Seattle, Washington 98195, USA. utrecht@u.washington.edu
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism*
Animals
Cytosol / metabolism
Dogs
Energy Metabolism
Homeostasis
Humans
Kinetics
Magnetic Resonance Spectroscopy
Mice
Mitochondria, Muscle / metabolism*
Muscle, Skeletal / metabolism
Oxidative Phosphorylation*
Oxygen Consumption
Proton-Translocating ATPases / metabolism
Signal Transduction*
Grant Support
ID/Acronym/Agency:
AR36281/AR/NIAMS NIH HHS; AR41793/AR/NIAMS NIH HHS; AR41928/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
58-64-0/Adenosine Diphosphate; EC 3.6.3.14/Proton-Translocating ATPases

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