Document Detail


A sialic acid-specific lectin from the mushroom Paecilomyces Japonica that exhibits hemagglutination activity and cytotoxicity.
MedLine Citation:
PMID:  15579126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mushroom Paecilomyces japonica, grown on the silkworm larvae, has been used in Asia as a nutraceutical, tea, and Chinese medicine. In the present study, a sialic acid-specific lectin has been purified from the mushroom P. japonica using affinity chromatography on a fetuin-agarose column. Electrophoretical analyses indicated that this lectin, designated P. japonica agglutinin (PJA), is an acidic protein with a molecular mass of 16 kDa, and has no intermolecular disulfide bonds. PJA induced hemagglutination activity in human ABO, mouse, rat, and rabbit erythrocytes. This activity was inhibited by sialic acid and sialoglycoproteins, but not by any other carbohydrates. PJA was stable at pH 4.0-8.0, and at temperatures below 55 degrees C. The activity of PJA was independent of EDTA and divalent cations. In addition, PJA exerts cytotoxic effects on the following cancer cell lines: human stomach cancer SNU-1, human pancreas cancer AsPc-1, and human breast cancer MDA-MB-231.
Authors:
Jee Hun Park; Chang Soo Ryu; Ha Na Kim; Young Jun Na; Hyun Joo Park; Hahyung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein and peptide letters     Volume:  11     ISSN:  0929-8665     ISO Abbreviation:  Protein Pept. Lett.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-06     Completed Date:  2005-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  563-9     Citation Subset:  IM    
Affiliation:
Physical Pharmacy Laboratory, College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-ku, Seoul 156-756, Korea. hahyung@cau.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophoresis, Polyacrylamide Gel
Erythrocytes / metabolism
Hemagglutination / physiology*
Humans
Hydrogen-Ion Concentration
Mice
N-Acetylneuraminic Acid / metabolism
Paecilomyces / metabolism*
Plant Lectins / metabolism*,  toxicity
Rabbits
Rats
Temperature
Chemical
Reg. No./Substance:
0/Plant Lectins; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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