| siRNA targeting survivin inhibits growth and induces apoptosis in human renal clear cell carcinoma 786-O cells. | |
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MedLine Citation:
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PMID: 19628339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the inhibitory effect of small interfering RNA (siRNA) targeting survivin gene on cell proliferation and apoptosis in human renal clear cell carcinoma 786-O cells. qRT-PCR, immunocytochemistry, and Western Blot were used to detect Survivin gene expression in 786-O cells. Cell proliferation was determined by BrdU assay and PCNA expression. Cell apoptosis was analyzed through detection of caspase-3 activity, and the effect of survivin-siRNA on Bcl-2 gene expression was also examined. Forty-eight hours after transfection, survivin expression was markedly inhibited at the mRNA and protein level. Downregulation of survivin resulted in a significant inhibition of tumor cell growth. Caspase-3 activity showed that siRNA targeting survivin gene induced cell apoptosis in 786-O cells. Moreover, Bcl-2 protein expression was markedly inhibited by transfection with siRNA against survivin. These results indicate that siRNA targeting survivin gene can downregulate survivin gene expression in 786-O cells, inhibit growth, and induce apoptosis of renal carcinoma cells. |
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Authors:
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Yong Zhang; Zhao-dian Chen; Chuan-jun Du; Gang Xu; Wei Luo |
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Publication Detail:
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Type: Journal Article Date: 2009-07-22 |
Journal Detail:
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Title: Pathology, research and practice Volume: 205 ISSN: 1618-0631 ISO Abbreviation: Pathol. Res. Pract. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-11-25 Completed Date: 2010-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7806109 Medline TA: Pathol Res Pract Country: Germany |
Other Details:
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Languages: eng Pagination: 823-7 Citation Subset: IM |
Affiliation:
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Department of Urology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Jiefang Rd#88, Hangzhou, Zhejiang Province 310009, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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genetics* Blotting, Western Carcinoma, Renal Cell / genetics*, metabolism, pathology Caspase 3 / metabolism Cell Line, Tumor Cell Proliferation* Down-Regulation Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Kidney Neoplasms / genetics*, metabolism, pathology Microtubule-Associated Proteins / genetics*, metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism RNA Interference* RNA, Messenger / metabolism RNA, Small Interfering / metabolism* Reverse Transcriptase Polymerase Chain Reaction Transfection |
| Chemical | |
Reg. No./Substance:
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0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/RNA, Small Interfering; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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