Document Detail

siRNA targeting survivin inhibits growth and induces apoptosis in human renal clear cell carcinoma 786-O cells.
MedLine Citation:
PMID:  19628339     Owner:  NLM     Status:  MEDLINE    
We investigated the inhibitory effect of small interfering RNA (siRNA) targeting survivin gene on cell proliferation and apoptosis in human renal clear cell carcinoma 786-O cells. qRT-PCR, immunocytochemistry, and Western Blot were used to detect Survivin gene expression in 786-O cells. Cell proliferation was determined by BrdU assay and PCNA expression. Cell apoptosis was analyzed through detection of caspase-3 activity, and the effect of survivin-siRNA on Bcl-2 gene expression was also examined. Forty-eight hours after transfection, survivin expression was markedly inhibited at the mRNA and protein level. Downregulation of survivin resulted in a significant inhibition of tumor cell growth. Caspase-3 activity showed that siRNA targeting survivin gene induced cell apoptosis in 786-O cells. Moreover, Bcl-2 protein expression was markedly inhibited by transfection with siRNA against survivin. These results indicate that siRNA targeting survivin gene can downregulate survivin gene expression in 786-O cells, inhibit growth, and induce apoptosis of renal carcinoma cells.
Yong Zhang; Zhao-dian Chen; Chuan-jun Du; Gang Xu; Wei Luo
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Publication Detail:
Type:  Journal Article     Date:  2009-07-22
Journal Detail:
Title:  Pathology, research and practice     Volume:  205     ISSN:  1618-0631     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  2009  
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  823-7     Citation Subset:  IM    
Department of Urology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Jiefang Rd#88, Hangzhou, Zhejiang Province 310009, China.
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MeSH Terms
Apoptosis / genetics*
Blotting, Western
Carcinoma, Renal Cell / genetics*,  metabolism,  pathology
Caspase 3 / metabolism
Cell Line, Tumor
Cell Proliferation*
Gene Expression Regulation, Neoplastic
Kidney Neoplasms / genetics*,  metabolism,  pathology
Microtubule-Associated Proteins / genetics*,  metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism
RNA Interference*
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/RNA, Small Interfering; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3

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