Document Detail


siRNA targeting LMP1-induced apoptosis in EBV-positive lymphoma cells is associated with inhibition of telomerase activity and expression.
MedLine Citation:
PMID:  16458115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epstein-Barr Virus (EBV) is closely associated with B cell malignancies. However, whether EBV appears to be absolutely required for cell proliferation and survival in lymphoma cells is still unknown. In this study, small interfering RNA (siRNA) targeting LMP1 was employed to investigate the effect of LMP1 on cell proliferation in EBV-positive lymphoblastoid B-cell line. A plasmid stable encoding 21-nt small RNA specifically and efficiently interfering LMP1 was constructed, resulting in a substantial loss of LMP1 mRNA and a significantly decreased LMP1 protein expression. Our data demonstrated that cell proliferation was completely inhibited and apoptosis was induced after knockdown of LMP1 gene in lymphoblastoid B-cell line. Also, we found that suppression of LMP1 caused downregulation of telomerase protein expression and decreased telomerase activity in lymphoma cells. In EBV-negative NPC cell line, transfection of plasmid expressing LMP1 greatly enhanced telomerase protein expression. Our results suggested that siRNA targeting LMP1 can induce apoptosis in EBV-positive lymphoma cells and is associated with inhibition of telomerase activity and expression. siRNA-directed LMP1 silencing may be of the therapeutic value for preventing and treating those EBV-associated tumors.
Authors:
Yu-Ping Mei; Xiao-Feng Zhu; Jun-Min Zhou; He Huang; Rong Deng; Yi-Xin Zeng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  232     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-06     Completed Date:  2006-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  189-98     Citation Subset:  IM    
Affiliation:
Department of Experimental Research, State Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-sen University, 651 DongFeng Road East, GuangZhou 510060, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Callithrix
Cell Line, Tumor
Cell Proliferation
DNA Damage
Herpesvirus 4, Human / isolation & purification*
Lymphoma / pathology,  therapy*,  virology*
Plasmids
RNA, Small Interfering / pharmacology*
Telomerase / antagonists & inhibitors*,  genetics,  metabolism
Transfection
Viral Matrix Proteins / antagonists & inhibitors*,  genetics,  physiology
Chemical
Reg. No./Substance:
0/EBV-associated membrane antigen, Epstein-Barr virus; 0/RNA, Small Interfering; 0/Viral Matrix Proteins; EC 2.7.7.49/Telomerase

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