Document Detail

siRNA-mediated knockdown of FoxP3 promotes the ratio of T-helper 1 (Th1) to Th2 in chronic hepatitis B patients.
MedLine Citation:
PMID:  22287403     Owner:  NLM     Status:  In-Data-Review    
To determine the effect of regulatory T cells on the ratio of Th1/Th2 differentiation in peripheral blood mononuclear cells of chronic hepatitis B patients, FoxP3, the essential transcription factor for Tcells differentiation and function, was knocked down by FoxP3 siRNA, which was affirmed by real-time polymerase chain reaction for decreased expression of FoxP3. Then, at different time points, comparing with control groups, we detected enhanced lymphocyte proliferation and up-regulated Th1-type cytokines, but down-regulated Th2-type cytokines by ELISA. Finally, to research the involved mechanisms, an increased ratio of T-bet/GATA-3 mRNA expression was found by real-time polymerase chain reaction. Our results suggest that siRNA-mediated knockdown of FoxP3 could regulate the immune balance of Th1/Th2 in chronic hepatitis B patients, which may be mediated partly by regulating transcription factors T-bet and GATA-3.
Yu Yongsheng; Li Xiaoliang; Tang Zhenghao; Zang Guoqing
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology     Volume:  22     ISSN:  1300-4948     ISO Abbreviation:  Turk J Gastroenterol     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9515841     Medline TA:  Turk J Gastroenterol     Country:  Turkey    
Other Details:
Languages:  eng     Pagination:  587-93     Citation Subset:  IM    
Sixth People's Hospital, Shanghai Jiaotong University, Department of Infectious Diseases, Shanghai, China.
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