| siRNA delivery by magnetofection. | |
| | |
MedLine Citation:
|
PMID: 18830925 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Magnetofection is defined as the magnetically enhanced delivery of nucleic acids associated with magnetic nanoparticles and has been utilized to deliver synthetic siRNAs to cultured cells. Certain magnetic nanomaterials associate with siRNAs and are suitable for siRNA delivery, either alone or in combination with cationic polymers or cationic lipid enhancers; these complexes are targeted to the cell surface by application of a gradient magnetic field. In this review methods are described to examine siRNA incorporation into magnetic complexes, to evaluate their magnetic responsiveness and to characterize their association with, and uptake into cells. These methods can be utilized to screen magnetic siRNA complexes for their suitability in functional siRNA delivery. Data, obtained since the first description of magnetofection in 2000, and novel results on the characterization of magnetic complexes containing synthetic siRNA are described. In addition, the benefits of siRNA delivery in vitro via magnetofection compared with standard non-magnetic methods of transfection using lipoplexes and polyplexes are highlighted. |
| | |
Authors:
|
Olga Mykhaylyk; Olivier Zelphati; Joseph Rosenecker; Christian Plank |
Related Documents
:
|
20732895 - Characterization of magnetic nanoparticles using programmed quadrupole magnetic field-f... 1947295 - Magnetic resonance techniques in musculoskeletal diseases. 20482195 - Quantized magnetic confinement in quantum wires. 18001995 - An improved quasi-static finite-difference scheme for induced field evaluation in mri b... 6292145 - Compensation techniques in nirs proton beam radiotherapy. 12034335 - Neural network-based segmentation of dynamic mr mammographic images. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
|
Title: Current opinion in molecular therapeutics Volume: 10 ISSN: 1464-8431 ISO Abbreviation: Curr. Opin. Mol. Ther. Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-10-02 Completed Date: 2008-11-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100891485 Medline TA: Curr Opin Mol Ther Country: England |
Other Details:
|
Languages: eng Pagination: 493-505 Citation Subset: IM |
Affiliation:
|
Technische Universität München, Institute of Experimental Oncology, Munich, Germany. olga.mykhaylyk@lrz.tu-muenchen.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Humans Magnetics* RNA, Small Interfering* Transfection / methods* |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Small Interfering |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gene therapy of cardiovascular disease.
Next Document: NT-501: an ophthalmic implant of polymer-encapsulated ciliary neurotrophic factor-producing cells.