Document Detail


A short-term culture method for the preparation of chromosome spreads from Rana pipiens.
MedLine Citation:
PMID:  327613     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosomes suitable for karyotyping were produced from short-term cultures of cells from embryonic northern leopard frogs, Rana pipiens, at various growth stages. Embryos were minced in a trypsin solution. The tissue fragments were subsequently cultured in an electrolytic solution containing fetal calf serum and Colcemid. We found this technique to be highly reproducible. In contrast to the conventional squash technique we were able to obtain a greater number of metaphase spreads suitable for identification and analysis of individual chromosomal aberrations.
Authors:
D J Picciano; R G McKinnell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  52     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-08-25     Completed Date:  1977-08-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  101-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Anura
Blood
Cells, Cultured
Chromosomes*
Colchicine
Cytological Techniques*
Karyotyping
Methods
Mitosis*
Rana pipiens
Chemical
Reg. No./Substance:
64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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