| The short-term consumption of a moderately high-fat diet alters nitric oxide bioavailability in lean female Zucker rats. | |
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MedLine Citation:
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PMID: 21539468 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To determine whether short-term consumption of a moderately high-fat diet (MHFD) affects nitric oxide (NO) production, the concentration of stable NO metabolites (NOx) in urine and plasma of rats fed a MHFD (15.6 %g fat) or control diet (4.5 %g fat) was measured weekly for 4 weeks. Plasma and urine NOx levels were significantly depressed in the MHFD group by week 1 and remained so for the duration of the study. Decreased NO bioavailability may result from a decrease in NO production or the scavenging of NO by reactive oxygen species (ROS). Because endothelial NOS (eNOS) is the major contributor to NO production and circulating levels of NOx, eNOS expression was measured in several tissues. At week 1, there was a MHFD-associated decrease in eNOS expression in the liver. Subsequently, eNOS expression declined in the heart and kidney medulla of MHFD-fed rats at weeks 3 and 4, respectively. The expression of eNOS in the kidney cortex and adipose tissue did not change. These results suggest that a MHFD alters eNOS expression in a time-dependent and tissue-specific manner. In the liver, NOS activity and tissue levels of NOx and nitrotyrosine were measured. Nitrotyrosine levels were used as an indirect measure of the NO scavenged by ROS. There was a decrease in NOS activity, suggesting that the low levels of hepatic NOx were due, in part, to a decrease in NO production. In addition, there was a dramatic increase in nitrotyrosine formation, suggesting that the decline in hepatic NOx was also due to an increased interaction of NO with ROS. Tyrosine nitration commonly has detrimental effects on proteins. The decrease in NO and increase in protein nitration could potentially have adverse effects on tissue function. |
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Authors:
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Kan Huang; Yue Huang; Jessica Frankel; Cassandra Addis; Lavina Jaswani; Paulette S Wehner; Elsa I Mangiarua; William D McCumbee |
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Publication Detail:
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Type: Journal Article Date: 2011-04-13 |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 89 ISSN: 1205-7541 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: Canada |
Other Details:
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Languages: eng Pagination: 245-57 Citation Subset: IM |
Affiliation:
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a Departments of Pharmacology, Physiology, and Toxicology, Marshall University, Joan C. Edwards School of Medicine, One John Marshall Drive, Huntington, WV 25755, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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