| A short method for the isolation of somatotrophs from the rat pituitary gland. | |
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MedLine Citation:
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PMID: 163739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Application of a density gradient centrifugation procedure for the isolation of somatotrophs from dispersed rat anterior pituitary cells is described. Two sequential discontinuous gradients of bovine serum albumin are used to enrich the somatotroph population from a frequency of 38% in the initial cell suspension to a final purity of 85%. The isolated somatotrophs retain both histochemical and ultrastructural integrity throughout the isolation procedure. The isolated somatotrophs also release immunoassayable growth hormone when incubated with 6 mM dibutyryl 3',5'-cyclic AMP. The method offers the advantages of being both simple and short. |
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Authors:
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G Snyder; W C Hymer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Endocrinology Volume: 96 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1975 Mar |
Date Detail:
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Created Date: 1975-06-09 Completed Date: 1975-06-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 792-6 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bucladesine / pharmacology Cell Separation / methods* Centrifugation, Density Gradient Growth Hormone / immunology, secretion Immunoassay Pituitary Gland / cytology* Pituitary Gland, Anterior / cytology*, secretion Rats Serum Albumin, Bovine |
| Chemical | |
Reg. No./Substance:
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0/Serum Albumin, Bovine; 362-74-3/Bucladesine; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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