| The short chain fatty acid sodium butyrate regulates the induction of CD1a in developing dendritic cells. | |
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MedLine Citation:
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PMID: 20851496 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Dendritic cells (DCs) are professional antigen-presenting cells with attributes for priming/activating T cells and mediating immune responses. Considering the importance of DCs in the initiation of immune responses, it will be of interest to study their mechanisms of regulation. Histone-modifying enzymes, such as histone deacetylases (HDACs), are critical in controlling chromatin organization. The aim of our study was to investigate DC differentiation under the influence of sodium butyrate (NaB), a short chain fatty acid that is a histone deacetylase inhibitor. Monocytes from healthy individuals were differentiated into immature DCs with IL-4 and GM-CSF in the presence or absence of NaB. DC differentiation was evaluated by CD14 and CD1a expression by flow cytometry. We observed that monocytes stimulated to differentiate in the presence of NaB displayed colony formation and dendritic cell morphology, lost CD14 and showed decreased secretion of IL-1β. The acquisition of CD1a, however, was impaired. Being a natural short chain fatty acid, NaB may regulate CD1a acquisition independently of its HDAC inhibitory activity. We observed that the addition of peroxisome proliferator-activated receptor γ (PPAR-γ) antagonist (GW9662) did not reverse NaB effect, suggesting this was not the pathway involved. On the other hand, CD1a can also be induced by toll like receptors 2 (TLR 2) agonists, such as Pam3Cys, and NaB inhibited this effect. Our data suggest that the histone deacetylase inhibitor NaB instead of impairing DC differentiation inhibits the acquisition of CD1a induced both by cytokines and by TLR 2 agonist stimulus. Furthermore, this occurs at the transcriptional level as NaB led to a decrease in mRNA levels of CD1a and upregulation of CD1d. |
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Authors:
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Clarissa Rodrigues Nascimento; Célio G Freire-de-Lima; Andréia da Silva de Oliveira; Franklin D Rumjanek; Vivian M Rumjanek |
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Publication Detail:
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Type: Journal Article Date: 2010-08-19 |
Journal Detail:
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Title: Immunobiology Volume: 216 ISSN: 1878-3279 ISO Abbreviation: Immunobiology Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002742 Medline TA: Immunobiology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 275-84 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier GmbH. All rights reserved. |
Affiliation:
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Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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