| The shock of the old: hydrodynamics for the masses. | |
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MedLine Citation:
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PMID: 18631155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrodynamic techniques such as analytical ultracentrifugation can provide key information about subunit stoichiometry and interaction strengths of protein-nucleic acid interactions. Analysis is complicated by (i) the need for low concentrations in order to observe both free and bound species and (ii) thermodynamic non-ideality. With the introduction of fluorescence optics, we are able to obtain data at lower concentrations, and improved understanding of the statistical thermodynamics of macromolecular solutions has allowed non-ideality to be accurately assigned. With these developments, it is possible now to assay protein-nucleic acid interactions at concentrations typically used in molecular biology assays. |
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Authors:
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David J Scott |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical Society transactions Volume: 36 ISSN: 0300-5127 ISO Abbreviation: Biochem. Soc. Trans. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-17 Completed Date: 2008-09-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506897 Medline TA: Biochem Soc Trans Country: England |
Other Details:
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Languages: eng Pagination: 766-70 Citation Subset: IM |
Affiliation:
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National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK. dj.scott@nottingham.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Nucleic Acids
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genetics,
metabolism Protein Binding Proteins / genetics, metabolism Thermodynamics Ultracentrifugation Water / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Proteins; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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