Document Detail

The sex-determining gene tra of Drosophila: molecular cloning and transformation studies.
MedLine Citation:
PMID:  3030733     Owner:  NLM     Status:  MEDLINE    
In Drosophila, the primary signal for sex determination is given by the ratio of X chromosomes to sets of autosomes (X:A). The primary signal is read by a key gene (Sxl) and transmitted down to the differentiation genes by the subordinate control genes tra, tra-2, ix and dsx. Mutations in tra transform chromosomal females (X/X; tra/tra) into sterile males (pseudomales). We have cloned the tra region by microdissection and chromosomal walking. We identified the gene using deficiency breakpoints, DNA aberrations in three different alleles of tra and by P-mediated transformation. A 3.8-kb fragment perfectly rescued the mutant phenotype of X/X; tra/tra flies, showing that it contained all the necessary information to restore female-specific functions in the mutant flies. We present evidence that most of the function of tra can be provided by a subsegment of 2 kb that is differentially transcribed or processed in males and females.
B Butler; V Pirrotta; I Irminger-Finger; R Nöthiger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The EMBO journal     Volume:  5     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-04-27     Completed Date:  1987-04-27     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3607-13     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Chromosome Mapping
Cloning, Molecular*
DNA Restriction Enzymes
Drosophila / genetics*,  physiology
Sex Determination (Analysis)
Transformation, Genetic*
X Chromosome
Grant Support
Reg. No./Substance:
EC 3.1.21.-/DNA Restriction Enzymes

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