Document Detail


A serum-free medium that supports the growth of cultured skeletal muscle satellite cells.
MedLine Citation:
PMID:  3905759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.
Authors:
R E Allen; M V Dodson; L S Luiten; L K Boxhorn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  21     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-01-14     Completed Date:  1986-01-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  636-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood
Cell Division / drug effects
Culture Media
Culture Techniques / methods
Dexamethasone / pharmacology
Fibroblast Growth Factors / pharmacology
Insulin / pharmacology
Kinetics
Linoleic Acid
Linoleic Acids / pharmacology
Muscles / cytology*,  drug effects
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
R01 AG03393/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Linoleic Acids; 11061-68-0/Insulin; 2197-37-7/Linoleic Acid; 50-02-2/Dexamethasone; 62031-54-3/Fibroblast Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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