Document Detail


The serine protease from rat liver and hepatoma 8999. Location and role in mitochondrial protein degradation.
MedLine Citation:
PMID:  209014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Hepatoma 8999 showed extremely high activity of serine protease, but similar activities of other lysosomal proteases to those of normal rat liver. 2. Serine protease from rat liver formed a single immunoprecipitation band against antiserum to purified protease from hepatoma 8999. 3. The serine proteases in rat liver and hepatoma 8999 were restricted to the inner membranes of the mitochondrial fraction. 4. Polyacrylamide gel electrophoresis with sodium dodecylsulfate showed that hepatoma 8999 mitochondria contained less of the slowest moving protein component than rat liver mitochondrial protein. This component was found to be the best substrate for mitochondrial serine protease in both liver and hepatoma 8999. 5. The role of serine protease in mitochondrial protein degradation is discussed on the basis of these results.
Authors:
Y Banno; H P Morris; N Katunuma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  83     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-15     Completed Date:  1978-09-15     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1545-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular / enzymology*
Cell Fractionation
Immunodiffusion
Kinetics
Liver Neoplasms / enzymology*
Mitochondria / enzymology*
Mitochondria, Liver / enzymology*
Neoplasm Proteins
Neoplasms, Experimental / enzymology
Peptide Hydrolases / metabolism*
Proteins
Rats
Serine
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Proteins; 56-45-1/Serine; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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