Document Detail


A serine carboxypeptidase gene (PsCP), expressed in early steps of reproductive and vegetative development in Pisum sativum, is induced by gibberellins.
MedLine Citation:
PMID:  12602875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cDNA clone encoding a serine carboxypeptidase (PsCP), isolated from young fruits of Pisum sativum L., was used to study the temporal and spatial expression and hormonal regulation of serine carboxypeptidase during reproductive and vegetative development. In unpollinated pea ovaries PsCP transcript levels decreased during senescence. However, during early fruit development, PsCP transcript were accumulated in both pericarp and seeds, preferentially in the nucellus, with a polar distribution at the chalazal region of the embryo sac, suggesting a role in seed development. PsCP transcript levels increased also when fruit set was induced in unpollinated ovaries by gibberellins, although the distribution was uniform. PsCP expression was also induced by auxins but not cytokinins, indicating a selective hormonal regulation of PsCP transcription. Localization of PsCP transcript after pollination parallel reported changes in gibberellin distribution, suggesting that PsCP transcription in developing fruits and seeds is induced by gibberellins. PsCP is also expressed in developing seedlings but not in cotyledons, suggesting that it is not involved in the mobilization of storage materials. PsCP transcripts were suppressed by treatment of seedlings with paclobutrazol and restored by gibberellic acid (GA3) treatment. In addition, PsCP transcript levels decreased in etiolated pea seedlings when they were exposed to continuous light but not when exposed to light in the presence of GA3. These results indicate that PsCP transcript accumulation is induced by gibberellins in developing seedlings. This is the first report of a serine carboxypeptidase-like gene induced by gibberellins in reproductive and vegetative developing tissues in dicotyledoneous plants.
Authors:
Manuel Cercós; Cristina Urbez; Juan Carbonell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  51     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-02-26     Completed Date:  2003-03-10     Revised Date:  2012-07-10    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  165-74     Citation Subset:  IM    
Affiliation:
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Carboxypeptidases / genetics*
Gene Expression Regulation, Developmental / drug effects,  radiation effects
Gene Expression Regulation, Enzymologic / drug effects,  radiation effects
Gene Expression Regulation, Plant / drug effects,  radiation effects
Gibberellins / pharmacology*
In Situ Hybridization
Light
Peas / enzymology,  genetics*,  growth & development
Plant Growth Regulators / pharmacology
RNA, Messenger / drug effects,  metabolism,  radiation effects
Reproduction / genetics
Seeds / enzymology,  genetics,  growth & development
Chemical
Reg. No./Substance:
0/Gibberellins; 0/Plant Growth Regulators; 0/RNA, Messenger; EC 3.4.-/Carboxypeptidases; EC 3.4.16.5/serine carboxypeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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