Document Detail


A serial dilution microfluidic device for cytotoxicity assays.
MedLine Citation:
PMID:  17946141     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel microfluidic device is presented which creates a linear serial dilution of two input fluid streams. This platform facilitates higher productivity as a component of a high throughput cytotoxicity testing strategy. A modeling solution is presented to create custom linear dilution schemes. The featured device creates a serial dilution of two solutions in the range of 1:9 through 9:1 across nine discrete dilutions. It has been validated to create a highly linear progression of dilutions with an R2 value of 0.9993. The device functions equivalently over a wide range of flow rates. The standard deviation of dilution values averages 0.76% over six flow rates spanning 0.5 to 16 microl min(-1).
Authors:
Adrian T O'Neill; Nancy Monteiro-Riviere; Glenn M Walker
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference     Volume:  1     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2006  
Date Detail:
Created Date:  2007-10-23     Completed Date:  2008-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2836-9     Citation Subset:  IM    
Affiliation:
Dept. of Biomed. Eng., North Carolina Univ., Chapel Hill, NC, USA. atoneill@ncsu.edu
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MeSH Terms
Descriptor/Qualifier:
Biological Assay / instrumentation*,  methods
Cell Culture Techniques / instrumentation*,  methods
Computer-Aided Design*
Equipment Design
Equipment Failure Analysis
Flow Cytometry / instrumentation*,  methods
Flow Injection Analysis / instrumentation*,  methods
Microfluidic Analytical Techniques / instrumentation*
Toxicity Tests / instrumentation*,  methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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