| A sensitive, rapid and specific determination of midazolam in human plasma and saliva by liquid chromatography/electrospray mass spectrometry. | |
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MedLine Citation:
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PMID: 15536636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid, sensitive and selective liquid chromatography/electrospray mass spectrometry (LC/ES-MS) method was developed for the quantitative determination of the anaesthetic benzodiazepine midazolam (MID) in human saliva and plasma from patients undergoing anesthesia procedures. Biological samples spiked with diazepam-d5, the internal standard, were extracted into diethyl ether. Compounds were separated on a Xterra RP18 column using a mobile phase of acetonitrile/formic acid 0.1% at a flow rate of 0.25 mL/min under a linear gradient. Column effluents were analyzed using MS with an ES source in the positive ionization mode. Calibration curves were linear in the concentration ranges of 1-250 and 0.2-25 ng/mL in plasma and saliva, respectively. The limits of detection were 0.5 ng/mL in plasma and 0.1 ng/mL in saliva, using a 0.5-mL sample volume. The recoveries of the spiked samples were above 65%. The method was applied to ten real samples from patients undergoing midazolam treatment. |
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Authors:
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Oscar Quintela; Angelines Cruz; Marta Concheiro; Ana de Castro; Manuel López-Rivadulla |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 18 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-12-03 Completed Date: 2005-03-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 2976-82 Citation Subset: IM |
Copyright Information:
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Copyright 2004 John Wiley & Sons, Ltd. |
Affiliation:
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Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calibration Chromatography, High Pressure Liquid / methods* Humans Midazolam / analysis*, blood*, chemistry Reference Standards Reproducibility of Results Saliva / chemistry* Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / methods* Time Factors |
| Chemical | |
Reg. No./Substance:
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59467-70-8/Midazolam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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