Document Detail


A sensitive and rapid method to determine the viability of freeze-dried bacterial cells.
MedLine Citation:
PMID:  12753251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To determine the potential use of flow cytometry for viability asssessment of freeze-dried bacterial cells. METHODS AND RESULTS: Escherichia coli CIP 54.8T and Vibrio metschnikovii CIP 104262 were analysed. The viability of freeze-dried cells resuspended in a nutrient broth was evaluated by culture whereas activity was determined by flow cytometry analysis of both esterase activity and cell death. Activity assessment by flow cytometry was found to be a rapid and good indicator of cell viability and was very efficient for quality control. For V. metschnikovii the fraction of active cells varies greatly depending on the freeze-drying procedure and within a given procedure. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Bacterial activity assessment by flow cytometry is very efficient for the control of freeze-dried cells.
Authors:
N Parthuisot; P Catala; P Lebaron; D Clermont; C Bizet
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  36     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2003  
Date Detail:
Created Date:  2003-05-19     Completed Date:  2003-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  412-7     Citation Subset:  IM    
Affiliation:
Observatoire Océanologique, Université Pierre et Marie Curie, CNRS UMR7621, Institut des Sciences de l'Univers, BP44, F-66651 Banyuls-sur-Mer Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Colony Count, Microbial
Culture Media
Escherichia coli / cytology,  growth & development*
Flow Cytometry / methods*
Fluorescent Dyes
Freeze Drying
Organic Chemicals*
Sensitivity and Specificity
Staining and Labeling / methods
Time Factors
Vibrio / cytology,  growth & development*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fluorescent Dyes; 0/Organic Chemicals; 0/SYBR Green II

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