| A sensitive and rapid bioassay of homogalacturonan synthase using 2-aminobenzamide-labeled oligogalacturonides. | |
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MedLine Citation:
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PMID: 12461139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polygalacturonate 4-alpha-galacturonosyltransferase (GalA T) activity was detected in the microsomal fraction isolated from pumpkin (Cucurbia moschata Duchesne, cv. Tokyou-Kabocha) seedlings using UDP-GalA and 2-aminobenzamide (2AB)-labeled oligogalacturonides. A 2AB-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (GalA) residues to give 2AB-labeled oligogalacturonides with a degree of polymerization (DP) between 12 and 17. Exogenous 2AB-labeled oligogalacturonide acceptors with a DP >3 are effective acceptor molecules for pumpkin GalA T. |
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Authors:
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Tadashi Ishii |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plant & cell physiology Volume: 43 ISSN: 0032-0781 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-12-03 Completed Date: 2003-03-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 1386-9 Citation Subset: IM |
Affiliation:
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Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, 305-8687 Japan. tishii@ffpri.affrc.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anthranilic Acids
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metabolism* Biological Assay / methods* Cucurbita / metabolism Glucuronosyltransferase Glycosyltransferases / metabolism* Microsomes / metabolism Oligosaccharides / metabolism* Plant Proteins / metabolism* Uridine Diphosphate Sugars / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anthranilic Acids; 0/Oligosaccharides; 0/Plant Proteins; 0/Uridine Diphosphate Sugars; 0/oligogalacturonic acid; 50722-58-2/UDP-galacturonic acid; 88-68-6/anthranilamide; EC 2.4.-/Glycosyltransferases; EC 2.4.1.17/Glucuronosyltransferase; EC 2.4.1.17/alpha-1,4-galacturonosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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