Document Detail

A sensitive and rapid bioassay of homogalacturonan synthase using 2-aminobenzamide-labeled oligogalacturonides.
MedLine Citation:
PMID:  12461139     Owner:  NLM     Status:  MEDLINE    
Polygalacturonate 4-alpha-galacturonosyltransferase (GalA T) activity was detected in the microsomal fraction isolated from pumpkin (Cucurbia moschata Duchesne, cv. Tokyou-Kabocha) seedlings using UDP-GalA and 2-aminobenzamide (2AB)-labeled oligogalacturonides. A 2AB-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (GalA) residues to give 2AB-labeled oligogalacturonides with a degree of polymerization (DP) between 12 and 17. Exogenous 2AB-labeled oligogalacturonide acceptors with a DP >3 are effective acceptor molecules for pumpkin GalA T.
Tadashi Ishii
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant & cell physiology     Volume:  43     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-12-03     Completed Date:  2003-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1386-9     Citation Subset:  IM    
Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, 305-8687 Japan.
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MeSH Terms
Anthranilic Acids / metabolism*
Biological Assay / methods*
Cucurbita / metabolism
Glycosyltransferases / metabolism*
Microsomes / metabolism
Oligosaccharides / metabolism*
Plant Proteins / metabolism*
Uridine Diphosphate Sugars / metabolism
Reg. No./Substance:
0/Anthranilic Acids; 0/Oligosaccharides; 0/Plant Proteins; 0/Uridine Diphosphate Sugars; 0/oligogalacturonic acid; 50722-58-2/UDP-galacturonic acid; 88-68-6/anthranilamide; EC 2.4.-/Glycosyltransferases; EC; EC,4-galacturonosyltransferase

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