Document Detail


A sensitive and rapid HPLC-ECD method for the simultaneous analysis of norepinephrine, dopamine, serotonin and their primary metabolites in brain tissue.
MedLine Citation:
PMID:  8298285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report details a very sensitive, rapid and accurate ion-pair HPLC-ECD method for the analysis of biogenic amines and their metabolites in brain tissue. The method described enables detection of picogram amounts of 3-methoxy-4-hydroxy phenethyleneglycol (MHPG) (37 pg), 3,4-dihydroxy phenylacetic acid (DOPAC) (18 pg), norepinephrine (NE) (12 pg), epinephrine (E) (6 pg), 5-hydroxyindoleacetic acid (5-HIAA) (18 pg), 3,4-dihydroxyphenylethylamine (DA) (6 pg), 3-methoxy-4-hydroxyphenylacetic acid (HVA) (12 pg) and 5-hydroxytryptamine (5-HT) (12 pg). The linearity of the method is from 18.75 ng/mL to 300 ng/mL for MHPG; 9.37 to 150 ng/mL for DOPAC and 5-HIAA; 6.25 to 100 ng/mL for NE, HVA and 5-HT; and from 3.12 to 100 ng/mL for E and DA. The reproducibility, expressed as coefficient of variance (CV%) within-run and between-run groups, was 2.25% and 19.49% for MHPG; 3.84% and 29.84% for DOPAC; 0.89% and 8.97% for NE; 1.26% and 5.61% for E; 1.07% and 28.77% for 5-HIAA; 2.65% and 10.65% for DA; 5.97% and 24.38% for HVA; and 4.44% and 9.45% for 5-HT.
Authors:
M E Alburges; N Narang; J K Wamsley
Related Documents :
10348315 - Normal ascorbic acid in cerebrospinal fluid of patients with infantile neuronal ceroid-...
16998855 - Amino acids in oviduct and uterine fluid and blood plasma during the estrous cycle in t...
9150615 - Cerebrospinal fluid neuropeptides and monoaminergic transmitters in patients with trige...
7514945 - Peripheral nerve stimulation increases serotonin and dopamine metabolites in rat spinal...
3488405 - Synthesis and antiinflammatory/analgesic activities of 11h-dibenzo[b, e,][1,4]dioxepina...
1254135 - The effect of 16,16-dimethyl prostaglandin e2 on meal-stimulated gastric acid secretion...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  7     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:    1993 Nov-Dec
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  306-10     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, North Dakota State University, College of Pharmacy, Fargo 58105.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biogenic Monoamines / analysis*
Brain Chemistry*
Chromatography, High Pressure Liquid
Dopamine / analysis
Electrochemistry
Indicators and Reagents
Male
Norepinephrine / analysis
Rats
Serotonin / analysis
Grant Support
ID/Acronym/Agency:
DA 05167/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Biogenic Monoamines; 0/Indicators and Reagents; 50-67-9/Serotonin; 51-41-2/Norepinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Metabolic fate of fenitrothion in liver, kidney and brain of rat.
Next Document:  High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues.