Document Detail

A sensitive and rapid HPLC-ECD method for the simultaneous analysis of norepinephrine, dopamine, serotonin and their primary metabolites in brain tissue.
MedLine Citation:
PMID:  8298285     Owner:  NLM     Status:  MEDLINE    
This report details a very sensitive, rapid and accurate ion-pair HPLC-ECD method for the analysis of biogenic amines and their metabolites in brain tissue. The method described enables detection of picogram amounts of 3-methoxy-4-hydroxy phenethyleneglycol (MHPG) (37 pg), 3,4-dihydroxy phenylacetic acid (DOPAC) (18 pg), norepinephrine (NE) (12 pg), epinephrine (E) (6 pg), 5-hydroxyindoleacetic acid (5-HIAA) (18 pg), 3,4-dihydroxyphenylethylamine (DA) (6 pg), 3-methoxy-4-hydroxyphenylacetic acid (HVA) (12 pg) and 5-hydroxytryptamine (5-HT) (12 pg). The linearity of the method is from 18.75 ng/mL to 300 ng/mL for MHPG; 9.37 to 150 ng/mL for DOPAC and 5-HIAA; 6.25 to 100 ng/mL for NE, HVA and 5-HT; and from 3.12 to 100 ng/mL for E and DA. The reproducibility, expressed as coefficient of variance (CV%) within-run and between-run groups, was 2.25% and 19.49% for MHPG; 3.84% and 29.84% for DOPAC; 0.89% and 8.97% for NE; 1.26% and 5.61% for E; 1.07% and 28.77% for 5-HIAA; 2.65% and 10.65% for DA; 5.97% and 24.38% for HVA; and 4.44% and 9.45% for 5-HT.
M E Alburges; N Narang; J K Wamsley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  7     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:    1993 Nov-Dec
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  306-10     Citation Subset:  IM    
Department of Pharmaceutical Sciences, North Dakota State University, College of Pharmacy, Fargo 58105.
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MeSH Terms
Biogenic Monoamines / analysis*
Brain Chemistry*
Chromatography, High Pressure Liquid
Dopamine / analysis
Indicators and Reagents
Norepinephrine / analysis
Serotonin / analysis
Grant Support
Reg. No./Substance:
0/Biogenic Monoamines; 0/Indicators and Reagents; 50-67-9/Serotonin; 51-41-2/Norepinephrine

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