Document Detail


A sensitive radioimmunoassay for fentanyl. Plasma level in dogs and man.
MedLine Citation:
PMID:  923629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antiserum to fentanyl was obtained in rabbits repeatedly injected with carboxyfentanyl conjugated to bovine serum albumin. Using the antiserum, a highly sensitive radioimmunoassay has been developed, based on the dextran-coated charcoal method. It proved possible to assay the drug directly in plasma, in amounts as small as 30 picogram in 0.5 ml. The antibody was highly specific for fentanyl and no cross-reaction was observed with its major metabolites. This sensitive and specific radioimmunoassay method was employed to determine fentanyl in plasma from six volunteers after an intravenous bolus of 0.2 mg, and in plasma from dogs treated both intravenously and subcutaneously with 0.02 mg/kg. The plasma level of fentanyl could be followed for up to 6 h after a therapeutic dose in dogs and man.
Authors:
M Michiels; R Hendriks; J Heykants
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of clinical pharmacology     Volume:  12     ISSN:  0031-6970     ISO Abbreviation:  Eur. J. Clin. Pharmacol.     Publication Date:  1977 Oct 
Date Detail:
Created Date:  1978-01-27     Completed Date:  1978-01-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1256165     Medline TA:  Eur J Clin Pharmacol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  153-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Antibodies / isolation & purification
Antibody Specificity
Dogs
Fentanyl / blood*,  immunology
Humans
Kinetics
Male
Methods
Rabbits / immunology
Radioimmunoassay
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies; 437-38-7/Fentanyl

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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