Document Detail


A sensitive method for the measurement of the novel pet endectocide, selamectin (UK-124,114), in dog and cat plasma by chemical derivatisation and high-performance liquid chromatography with fluorescence detection.
MedLine Citation:
PMID:  11108544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An analytical method has been developed for the novel pet endectocide, selamectin (USAN, UK-124, 114), in dog and cat plasma to facilitate pharmacokinetic profiling for this compound. The method involves solid phase extraction of the compound and internal standard from plasma followed by chemical derivatisation using triethylamine and trifluoroacetic anhydride. This reaction yields a highly fluorescent product and thus provides a sensitive assay. Using a sample volume of 1.0 ml for dog plasma the assay has been validated over a concentration range of 0.2-40 ng/ml. Due to smaller plasma volumes for cat plasma samples, the assay was validated over a concentration range of 1.0- 200 ng/ml using a sample volume of 0.2 ml. The analyte has been shown to be stable for 48 h at room temperature and through three freeze thaw cycles in dog plasma. The analytical method is highly specific and proved suitable for the analysis of selamectin in dog and cat plasma samples following doses of compound by parenteral and non-parenteral routes.
Authors:
D K Walker; K S Fenner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  24     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-05-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  105-11     Citation Subset:  IM    
Affiliation:
Department of Drug Metabolism, Pfizer Central Research, Sandwich, Kent, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiparasitic Agents / blood*
Cats
Chromatography, High Pressure Liquid
Dogs
Drug Stability
Fluorescence
Ivermectin / analogs & derivatives*,  blood,  chemistry
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antiparasitic Agents; 0/selamectin; 70288-86-7/Ivermectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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