Document Detail


A sensitive high-performance liquid chromatographic assay for melphalan and its hydrolysis products in blood and plasma.
MedLine Citation:
PMID:  3349564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive high-performance liquid chromatographic assay has been developed for the measurement of the alkylating cytostatic drug melphalan (4-[bis(2-chloroethyl)amino]-L-phenyl-alanine, or L-phenylalanine-mustard, L-PAM) and its two hydrolysis products, monohydroxy melphalan (MOH) and dihydroxy melphalan (DOH). A reversed-phase phenyl column and a mobile phase consisting of acetonitrile/citrate buffer made possible an isocratic separation and quantification. N,N-[bis(2-hydroxy-ethyl)]toluidine has been synthesized as an internal standard structurally related to DOH. A new, accurate "kinetic" calibration procedure enabled us to determine even the concentration of the unstable MOH. The lower limit of quantification was 30 ng/ml for L-PAM and 20 ng/ml for both DOH and MOH with fluorescence detection. The use of this method is illustrated by some pharmacokinetic data in systemic and locoregional melphalan therapy.
Authors:
H K Osterheld; E Musch; G E von Unruh; U Loos; H Rauschecker; B J Mühlenbruch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer chemotherapy and pharmacology     Volume:  21     ISSN:  0344-5704     ISO Abbreviation:  Cancer Chemother. Pharmacol.     Publication Date:  1988  
Date Detail:
Created Date:  1988-05-11     Completed Date:  1988-05-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7806519     Medline TA:  Cancer Chemother Pharmacol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  156-62     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Bonn, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Calibration
Chemical Phenomena
Chemistry
Chromatography, High Pressure Liquid
Humans
Hydrolysis
Melphalan / blood*,  pharmacokinetics
Predictive Value of Tests
Quality Control
Chemical
Reg. No./Substance:
148-82-3/Melphalan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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