Document Detail


A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate.
MedLine Citation:
PMID:  22113359     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In pH 8.9 Tris-HCl buffer solutions, alkaline phosphatase (ALP) catalyzed the hydrolysis of ascorbic acid 2-phosphate (AAP) substrate to form ascorbic acid. Then H(3)PO(4) was added to stop the enzymatic reaction and HAuCl(4) was used to react with ascorbic acid to generate gold nanoparticles that exhibited a resonance scattering (RS) peak at 600 nm. Under the selected conditions, when the activity of ALP increased, the formed ascorbic acid and gold nanoparticles also increased. Thus, the RS intensity at 600 nm enhanced linearly. The linear range was 0.06-22 U/L, with a detection limit of 0.03 U/L. The ALP in serum was analyzed, and the results were in agreement with those of the fluorescence method.
Authors:
Zhiliang Jiang; Meng Wu; Gaosan Liu; Aihui Liang
Related Documents :
6699649 - Sialic acid and fatty acid concentrations in lymphocytes, red blood cells and plasma fr...
9406769 - Biochemical properties of a neuraminidase of trichomonas vaginalis.
9222879 - Serum sialic acid concentration and atherosclerotic risk factors.
3723289 - Identification of sialic acids on the cell surface of hyphae and conidia of the human p...
7864379 - Alpha 2-8 sialic acid polymers: size, structure, and compositional analysis.
13723329 - Specific inhibition of rho(d) antibody by sialic acids.
10847609 - Observation of tetrahydrofolylpolyglutamic acid in bacteria cells by matrix-assisted la...
16715619 - Hypertension as a low-grade systemic inflammatory condition that has its origins in the...
11779119 - An improved fluorometric high-performance liquid chromatography method for sialic acid ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-24
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  -     ISSN:  1615-7605     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guangxi Normal University, Guilin, 541004, China, zljiang@mailbox.gxnu.edu.cn.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Plasma thrombin-cleaved osteopontin elevation after carotid artery stenting in symptomatic ischemic ...
Next Document:  Evidences for Antinociceptive Effect of 17-Alpha-Hydroxyprogesterone Caproate in Carpal Tunnel Syndr...