| The sensitive determination of nucleic acids using a fluorescence-quenching method. | |
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MedLine Citation:
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PMID: 16645957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experiments indicated that nucleic acids can quench the fluorescence of the Eu3+ -2-thenoyltrifluoroacetone (TTA)-1,10-phenanthroline (Phen) system. Based on this, a sensitive method for the determination of nucleic acids was proposed. The experiments indicated that under the optimum conditions, the quenched fluorescence intensity was in proportion to the concentration of nucleic acids in the range 1.0 x 10(-11)-1.0 x 10(-6) g/mL for yeast RNA (yRNA), 5.0 x 10(-11)-5.0 x 10(-7) g/mL for fish sperm (fsDNA) and 1.0 x 10(-10)-1.5 x 10(-6) g/mL for calf thymus DNA (ctDNA). Their detection limits were 3.0 x 10(-12), 4.0 x 10(-12) and 5.0 x 10(-11) g/mL, respectively. Therefore, the proposed method is one of the most sensitive methods available. The interaction between nucleic acids and Eu3+ -TTA-Phen is also discussed. |
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Authors:
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Zhen Jia; Jinghe Yang; Xia Wu; Changxia Sun; Fei Wang; Shufang Liu; Feng Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Luminescence : the journal of biological and chemical luminescence Volume: 21 ISSN: 1522-7235 ISO Abbreviation: Luminescence Publication Date: 2006 Jul-Aug |
Date Detail:
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Created Date: 2006-08-23 Completed Date: 2006-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100889025 Medline TA: Luminescence Country: England |
Other Details:
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Languages: eng Pagination: 207-13 Citation Subset: IM |
Copyright Information:
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Copyright 2006 John Wiley & Sons, Ltd. |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry of the Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Europium
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chemistry Hydrogen-Ion Concentration Nucleic Acids / analysis*, chemistry Organometallic Compounds / chemistry Phenanthrolines / chemistry Sensitivity and Specificity Solutions / chemistry Spectrometry, Fluorescence / methods* Thenoyltrifluoroacetone / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Organometallic Compounds; 0/Phenanthrolines; 0/Solutions; 326-91-0/Thenoyltrifluoroacetone; 66-71-7/1,10-phenanthroline; 7440-53-1/Europium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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