Document Detail


A sensitive, convenient radioimmunoassay procedure which demonstrates that serum hTSH is suppressed below the normal range in thyrotoxic patients.
MedLine Citation:
PMID:  520279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly sensitive radioimmunoassay procedures for the measurement of serum hTSH is described which permits delineation of the entire range of values in normal subjects (0.5-4.5 microU/ml). The procedure involves the concentration of hTSH from serum by adsorption to concanavalin A covalently bound to 4B Sepharose, Lactoperoxidase iodination of hTSH, and disequilibrium assay conditions. This method utilizes commonly available radioimmunoassay materials and is convenient to perform. Our results with this assay show that patients with thyrotoxicosis of a variety of etiologies have serum hTSH levels suppressed well below the normal range.
Authors:
R E Wehmann; H A Rubenstein; B C Nisula
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrine research communications     Volume:  6     ISSN:  0093-6391     ISO Abbreviation:  Endocr Res Commun     Publication Date:  1979  
Date Detail:
Created Date:  1980-03-27     Completed Date:  1980-03-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0426337     Medline TA:  Endocr Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Chromatography, Gel
Concanavalin A / diagnostic use
Humans
Hyperthyroidism / blood*
Immune Sera
Iodine / diagnostic use
Middle Aged
Radioimmunoassay / methods
Thyrotropin / blood*
Chemical
Reg. No./Substance:
0/Immune Sera; 11028-71-0/Concanavalin A; 7553-56-2/Iodine; 9002-71-5/Thyrotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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