Document Detail

A sensitive amperometric immunosensor for alpha-fetoprotein based on carbon nanotube/DNA/Thi/nano-Au modified glassy carbon electrode.
MedLine Citation:
PMID:  20627666     Owner:  NLM     Status:  MEDLINE    
A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using films of multi-wall carbon nanotubes/DNA/thionine/gold nanoparticles (nano-Au). Firstly, multiwall carbon nanotubes (MWCNT) dispersed in poly(diallydimethlammonium chloride) (PDDA) were immobilized on the nano-Au film which was electrochemically deposited on the surface of glassy carbon electrode. Then a negatively charged DNA film was absorbed on the positively charged PDDA. Subsequently, thionine was attached to the electrode via the electrostatic interaction between thionine and the DNA. Finally, the nano-Au was retained on the thionine film for immobilization of AFP antibody (anti-AFP). The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensors were studied in detail. Under optimal conditions, the proposed immunosensor exhibited good electrochemical behavior to AFP in a two concentration ranges: 0.01-10.0 and 10.0-200.0 ng/mL with a relatively low detection limit of 0.04 ng/mL at three times the background noise. Moreover, the selectivity, repeatability and stability of the proposed immunosensor were acceptable.
Xiao-Qi Ran; Ruo Yuan; Ya-Qin Chai; Cheng-Lin Hong; Xiao-Qing Qian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-16     Completed Date:  2010-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  421-6     Citation Subset:  IM    
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
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MeSH Terms
Biosensing Techniques / instrumentation*
Carbon / chemistry
DNA / chemistry*,  ultrastructure
Electrochemical Techniques / instrumentation*
Glass / chemistry
Gold / chemistry*
Hydrogen-Ion Concentration
Metal Nanoparticles / chemistry*,  ultrastructure
Nanotubes, Carbon / chemistry*
Phenothiazines / chemistry
Reproducibility of Results
Time Factors
alpha-Fetoproteins / immunology*
Reg. No./Substance:
0/Buffers; 0/Nanotubes, Carbon; 0/Phenothiazines; 0/Solutions; 0/alpha-Fetoproteins; 581-64-6/thionine; 7440-44-0/Carbon; 7440-57-5/Gold; 9007-49-2/DNA; 91080-16-9/calf thymus DNA

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