Document Detail


A sensitive amperometric immunosensor for alpha-fetoprotein based on carbon nanotube/DNA/Thi/nano-Au modified glassy carbon electrode.
MedLine Citation:
PMID:  20627666     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using films of multi-wall carbon nanotubes/DNA/thionine/gold nanoparticles (nano-Au). Firstly, multiwall carbon nanotubes (MWCNT) dispersed in poly(diallydimethlammonium chloride) (PDDA) were immobilized on the nano-Au film which was electrochemically deposited on the surface of glassy carbon electrode. Then a negatively charged DNA film was absorbed on the positively charged PDDA. Subsequently, thionine was attached to the electrode via the electrostatic interaction between thionine and the DNA. Finally, the nano-Au was retained on the thionine film for immobilization of AFP antibody (anti-AFP). The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensors were studied in detail. Under optimal conditions, the proposed immunosensor exhibited good electrochemical behavior to AFP in a two concentration ranges: 0.01-10.0 and 10.0-200.0 ng/mL with a relatively low detection limit of 0.04 ng/mL at three times the background noise. Moreover, the selectivity, repeatability and stability of the proposed immunosensor were acceptable.
Authors:
Xiao-Qi Ran; Ruo Yuan; Ya-Qin Chai; Cheng-Lin Hong; Xiao-Qing Qian
Related Documents :
17450936 - Enhanced oxidation of estrone at multi-wall carbon nanotubes film electrode: direct evi...
16937176 - Confounding factors in water emg recordings: an approach to a definitive standard.
3673776 - Microelectronic sensors for simultaneous measurement of po2 and ph.
19265426 - Scanning micropipet contact method for high-resolution imaging of electrode surface red...
9056336 - Volumetric mixing in anionic/nonionic, cationic/nonionic, and anionic/cationic mixed mi...
12918046 - Synthesis and characterization of a novel polymer-ceramic system for biodegradable comp...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-16     Completed Date:  2010-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  421-6     Citation Subset:  IM    
Affiliation:
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / instrumentation*
Buffers
Calibration
Carbon / chemistry
Cattle
DNA / chemistry*,  ultrastructure
Electrochemical Techniques / instrumentation*
Electrodes
Glass / chemistry
Gold / chemistry*
Hydrogen-Ion Concentration
Metal Nanoparticles / chemistry*,  ultrastructure
Nanotubes, Carbon / chemistry*
Phenothiazines / chemistry
Reproducibility of Results
Solutions
Temperature
Time Factors
alpha-Fetoproteins / immunology*
Chemical
Reg. No./Substance:
0/Buffers; 0/Nanotubes, Carbon; 0/Phenothiazines; 0/Solutions; 0/alpha-Fetoproteins; 581-64-6/thionine; 7440-44-0/Carbon; 7440-57-5/Gold; 9007-49-2/DNA; 91080-16-9/calf thymus DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular implications in the nanoencapsulation of the anti-tuberculosis drug rifampicin within flow...
Next Document:  Direct spectroscopic observation of binding of sugars to polymers having phenylboronic acids substit...