| The sensing of essential amino acid deficiency in the anterior piriform cortex, that requires the uncharged tRNA/GCN2 pathway, is sensitive to wortmannin but not rapamycin. | |
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MedLine Citation:
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PMID: 19800362 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Animals detect and reject their first essential/indispensable amino acid (IAA) deficient meal within 20min; this IAA sensing requires an intact anterior piriform cortex (APC). In the biochemical responses to IAA deficiency in the APC we have shown that: uncharged tRNA is the primary sensor; IAA transport is increased; and signaling, including the extracellular-regulated kinase (ERK1/2), is activated. The mammalian target of rapamycin (mTOR) is a potential AA sensor and is regulated by AA transport. Previously, the inhibitors, rapamycin for mTOR, wortmannin for phosphoinositide 3 kinase (PI3K) and PD98059 for ERK, each blocked the upregulation of the System A transporter in IAA depleted APC neurons. Here we injected these same inhibitors into the APC and measured intake of an IAA deficient diet. Rapamycin had no effect on the rejection of the IAA deficient diet, but wortmannin increased ERK activation and intake of the deficient diet before 40min and PD98059 acted after 40min to increase the second meal. While the specific wortmannin target involved in blocking the behavioral response remains unclear, we conclude that mTOR is dispensable for sensing IAA deficiency in the APC, and that ERK is associated with the secondary learned responses to IAA deficient diets. |
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Authors:
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S Hao; C M Ross-Inta; D W Gietzen |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-10-01 |
Journal Detail:
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Title: Pharmacology, biochemistry, and behavior Volume: 94 ISSN: 1873-5177 ISO Abbreviation: Pharmacol. Biochem. Behav. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0367050 Medline TA: Pharmacol Biochem Behav Country: United States |
Other Details:
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Languages: eng Pagination: 333-40 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis CA 95616, USA. haos@ajiusa.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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NS04321/NS/NINDS NIH HHS; NS33347/NS/NINDS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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