Document Detail


A semiautomated method for erythrocyte antigen typing on microtitration plates.
MedLine Citation:
PMID:  1448965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a method for erythrocyte antigen typing on U-bottom microtitration plates. This method permitted phenotyping of erythrocytes by an enzyme-enhanced hemagglutination assay simultaneously with an indirect antihuman globulin assay within the same microtitration plate. A robotic sample processor identified the samples, prepared the red cell suspensions and distributed the suspension along with the diluted antisera onto the microtitration plates. The assay results were read and interpreted using a microtitration plate photometer controlled by software specially designed to interpret agglutination reactions. Sample identities and assay results were automatically connected by the computer system and transmitted to a minicomputer system.
Authors:
S Sallander; S Pegert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vox sanguinis     Volume:  63     ISSN:  0042-9007     ISO Abbreviation:  Vox Sang.     Publication Date:  1992  
Date Detail:
Created Date:  1992-12-29     Completed Date:  1992-12-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  215-9     Citation Subset:  IM    
Affiliation:
Stockholm Blood Transfusion Service, Södersjukhuset, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Automation
Blood Grouping and Crossmatching*
Coombs' Test
Erythrocytes / immunology*
Humans
Papain
Phenotype
Rh-Hr Blood-Group System / immunology
Titrimetry
Chemical
Reg. No./Substance:
0/Rh-Hr Blood-Group System; EC 3.4.22.2/Papain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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