Document Detail

A semianalytical model to study the effect of cortical tension on cell rolling.
MedLine Citation:
PMID:  21156128     Owner:  NLM     Status:  MEDLINE    
Cell rolling on the vascular endothelium plays an important role in trafficking of leukocytes, stem cells, and cancer cells. We describe a semianalytical model of cell rolling that focuses on the microvillus as the unit of cell-substrate interaction and integrates microvillus mechanics, receptor clustering, force-dependent receptor-ligand kinetics, and cortical tension that enables incorporation of cell body deformation. Using parameters obtained from independent experiments, the model showed excellent agreement with experimental studies of neutrophil rolling on P-selectin and predicted different regimes of cell rolling, including spreading of the cells on the substrate under high shear. The cortical tension affected the cell-surface contact area and influenced the rolling velocity, and modulated the dependence of rolling velocity on microvillus stiffness. Moreover, at the same shear stress, microvilli of cells with higher cortical tension carried a greater load compared to those with lower cortical tension. We also used the model to obtain a scaling dependence of the contact radius and cell rolling velocity under different conditions of shear stress, cortical tension, and ligand density. This model advances theoretical understanding of cell rolling by incorporating cortical tension and microvillus extension into a versatile, semianalytical framework.
Suman Bose; Sarit K Das; Jeffrey M Karp; Rohit Karnik
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-15     Completed Date:  2011-03-28     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3870-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
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MeSH Terms
Biomechanics / physiology
Leukocyte Rolling / physiology*
Microvilli / metabolism
Models, Biological*
P-Selectin / metabolism
Stress, Mechanical*
Surface Properties
Time Factors
Grant Support
Reg. No./Substance:
0/Ligands; 0/P-Selectin

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