| A semi-pilot-scale procedure for isolating and purifying soybean (Glycine max) lectin. | |
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MedLine Citation:
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PMID: 14705873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Availability of gram quantities of purified soybean lectin (SBL) to scientists will foster discovery of novel biomedical applications of the lectin and provide the opportunity to investigate the antinutritional effects of SBL in soybean-consuming food animals and poultry. Therefore, a semi-pilot-scale procedure for isolating and purifying SBL was designed. Defatted soyflour was extracted overnight with 0.9% NaCl at 4 degrees C. The extract obtained was filtered (0.45 microm membrane) and subjected to affinity chromatography using a column containing N-acetyl-D-galactosamine resin that is specific for SBL. Bound SBL was eluted off the column with 0.14 M galactose solution. The eluent was ultrafiltered (30 kDa), and the resulting solution (SBL and water) was freeze-dried. Electrophoretic analysis and hemagglutination assay revealed that the freeze-dried SBL was similar to Sigma-grade SBL in purity and activity (35 and 33 HU/mg protein, respectively). The procedure yielded 141 mg of SBL/100 g of soyflour. |
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Authors:
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Yewande O Fasina; Harold E Swaisgood; Jim D Garlich; Henry L Classen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 51 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2004-01-06 Completed Date: 2005-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 4532-8 Citation Subset: IM |
Affiliation:
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Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon S7N 5A8, Saskatchewan, Canada. fasinyo@acesag.auburn.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Pilot Projects Plant Lectins / isolation & purification* Soybean Proteins / isolation & purification* Soybeans / chemistry Ultrafiltration |
| Chemical | |
Reg. No./Substance:
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0/Plant Lectins; 0/Soybean Proteins; 0/soybean lectin |
| Comments/Corrections | |
Comment In:
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J Agric Food Chem. 2004 May 19;52(10):3208; author reply 3209
[PMID:
15137877
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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