Document Detail


A semi-automated vibriocidal assay for improved measurement of cholera vaccine-induced immune responses.
MedLine Citation:
PMID:  17888533     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vibriocidal antibody assay has been a surrogate standard assay in the evaluation of cholera vaccine efficacy because it has a good correlation with protection. Although the optical density-based vibriocidal assay in a 96-well microtiter-plate format is widely used in clinical trials, it has limitations as vibriocidal titers are altered by incubation time and samples with the same end-point titers could have potentially different vibriocidal kinetics. In the present study, we developed an improved agar-plate assay coupled with an automated colony counting system. Through testing 30 pairs of human sera from vaccinees administered with a cholera vaccine or placebo, these two assays showed good correlations for the vibriocidal titers and fold increases in titers between pre- and post-vaccinated sera as determined by the Pearson correlation coefficient and the Regression coefficient. Notably, the newly-developed semi-automated assay demonstrated that serum samples with the same end-point titers turned out to have distinct vibriocidal kinetics that were not distinguishable by the microtiter-plate assay. The semi-automated assay responded specifically to Vibrio cholerae but not to irrelevant bacteria such as Salmonella typhi and Escherichia coli. These results demonstrate that the semi-automated assay provides better sensitivity, accuracy, and stability of the assay results with minimized efforts than conventional microtiter-plate assay and could provide a useful tool as an in vitro surrogate assay for the evaluation of cholera vaccine efficacy.
Authors:
Jae Seung Yang; Hye Jin Kim; Cheol-Heui Yun; Seok-Seong Kang; Jintaek Im; Hak-Sung Kim; Seung Hyun Han
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-31
Journal Detail:
Title:  Journal of microbiological methods     Volume:  71     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-15     Completed Date:  2008-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  141-6     Citation Subset:  IM    
Affiliation:
Laboratory Sciences Division, International Vaccine Institute, Seoul 151-600, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / blood,  immunology
Cholera / prevention & control
Cholera Vaccines / immunology*
Colony Count, Microbial
Escherichia coli / immunology
Humans
Immunoassay / methods*
Microbial Viability / immunology*
Salmonella typhi / immunology
Sensitivity and Specificity
Serum / immunology
Vibrio cholerae / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Cholera Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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