Document Detail

A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein.
MedLine Citation:
PMID:  22535306     Owner:  NLM     Status:  Publisher    
In this study, we report a facile method for the preparation of core-shell magnetic molecularly imprinted polymers (MIPs) for protein recognition. Uniform carboxyl group functionalized Fe(3)O(4) nanoparticles (NPs) were synthesized using a solvothermal method. Magnetic MIPs were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the Fe(3)O(4) NPs. A thin layer of polydopamine can be coated on Fe(3)O(4) NPs via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of Fe(3)O(4) NPs and dopamine. More importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. The as-prepared lysozyme-imprinted Fe(3)O(4)@polydopamine NPs show high binding capacity and acceptable specific recognition behavior towards template proteins. This method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis.
Min Zhang; Xihao Zhang; Xiwen He; Langxing Chen; Yukui Zhang
Related Documents :
22549896 - A selenium-based click adomet analogue for versatile substrate labeling with wild-type ...
17475766 - Genetic and antigenic diversity of the surface protective antigen proteins of erysipelo...
7849616 - Immunization of mice with proteins reacted with malonic dialdehyde (mda): comparison be...
18372626 - Detection of cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies...
18181316 - Protein depletion in thai patients with hip fractures.
23970546 - Mitochondrial protein bmpapi modulates the length of mature pirnas.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-25
Journal Detail:
Title:  Nanoscale     Volume:  -     ISSN:  2040-3372     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
State Key Laboratory of Medical Chemical Biology, Nankai University, Tianjin 300071, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Multiple substance use and self-reported suicide attempts by adolescents in 16 European countries.
Next Document:  Cocaine self-administration behaviors in Clock?19 mice.