Document Detail

The selenium requirement for glutathione peroxidase mRNA level is half of the selenium requirement for glutathione peroxidase activity in female rats.
MedLine Citation:
PMID:  8814215     Owner:  NLM     Status:  MEDLINE    
To determine critically the selenium (Se) requirement for weanling female rats, we used glutathione peroxidase (GSH: H2O2 oxidoreductase, EC (GPX) mRNA and a number of other parameters to assess Se status. Rats were fed a basal torulayeast diet (0.007 micrograms Se/g) supplemented with Se as Na2SeO3 in graded levels from 0 to 0.3 micrograms Se/g diet for 32 d (3 rats/group). Selenium supplementation had no effect on growth, showing that the Se requirement for growth is less than 0.007 micrograms Se/g diet, whereas other parameters showed significant increases with Se supplementation. In rats fed the Se-deficient basal diet, liver Se concentration was 4 +/- 0%, plasma GPX activity was 8 +/- 1%, erythrocyte GPX activity was 40 +/- 3%, liver GPX activity was 2 +/- 1%, and liver GPX mRNA levels were 11-17% of the levels in rats fed 0.1 micrograms Se/g diet. Liver Se concentration and GPX activity in plasma, erythrocytes and liver all reached a plateau breakpoint at or near 0.1 micrograms Se/g diet, indicating that the dietary Se requirement for maximal GPX activity in growing female rats is 0.1 micrograms Se/g diet. Liver GPX mRNA levels reached the plateau breakpoint at 0.05 micrograms Se/g diet, showing that the minimum dietary Se requirement for maximal GPX mRNA levels in female rats is half of the Se requirement for maximal GPX activity. This experiment demonstrates that GPX mRNA can be used to determine the dietary Se requirement; the gap between the dietary Se necessary for maximal GPX mRNA and that for maximal GPX activity may represent an evolutionarily derived biological margin of safety.
S L Weiss; J K Evenson; K M Thompson; R A Sunde
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  126     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-29     Completed Date:  1996-10-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2260-7     Citation Subset:  IM    
Nutritional Sciences Group, University of Missouri, Columbia 65211, USA.
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MeSH Terms
Analysis of Variance
Blotting, Northern
Body Weight / physiology
Cytosol / enzymology
Erythrocytes / enzymology
Food, Fortified
Gene Expression Regulation, Enzymologic / drug effects,  physiology
Glutathione Peroxidase / analysis*,  drug effects,  genetics*
Liver / anatomy & histology,  chemistry,  enzymology
Nutritional Requirements
Organ Size
RNA, Messenger / analysis*,  genetics,  metabolism
Random Allocation
Selenium / administration & dosage,  pharmacology*,  physiology
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 7782-49-2/Selenium; EC Peroxidase

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