Document Detail


A selective metabolite array for the detection of phosphometabolites.
MedLine Citation:
PMID:  22483219     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Immobilised metal ion affinity (IMA) has been traditionally used specifically for the separation of phosphorylated proteins and nucleic acids, in proteomics and genomics, respectively. This report describes the novel application of IMA in metabolomics for the development of metabolite arrays to detect phosphometabolites using a plasma polymer-modified surface. Immobilisation of gallium, zirconium, cobalt, copper, zinc, nickel, iron, and chromium to acrylic acid plasma polymer followed by subsequent exposure to metabolites (phospho- and non-phosphometabolites) was investigated. Results analysed using ToF-SIMS suggests that gallium and zirconium exhibit higher phosphometabolite affinity and specificity compared to other metals, and can be used to develop metabolite arrays for the detection of phosphometabolites.
Authors:
Malinda Salim; Gregory J S Fowler; Phillip C Wright; Seetharaman Vaidyanathan
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Publication Detail:
Type:  Journal Article     Date:  2012-03-03
Journal Detail:
Title:  Analytica chimica acta     Volume:  724     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  119-26     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, S1 3JD Sheffield, UK.
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