Document Detail

A selective effect of Ni2+ on wave initiation in bull sperm flagella.
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MedLine Citation:
PMID:  7430248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bull sperm that are extracted with 0.1% Triton X-100 and restored to motility with Mg2+-ATP lose coordination and stop swimming in the presence of 0.5 mM NiSO4. Although spontaneous coordination of flagellar waves is lost after exposure to Ni2+, other functions of the flagellum remain intact. The capacity for wave propagation along the flagellum is maintained together with the capacity for microtubular sliding. Wave motility can be restored to Ni2+-inhibited sperm by inducing a permanent bend onto the flagellum by micromanipulation. In the absence of such intervention, the loss of wave coordination is complete and irreversible. Ni2+-inhibited demembranated cells that are kept active by maintaining a bend in the flagellum exhibit a normal beat frequency. Both intact and demembranated sperm can retain spontaneous wave production at considerably slower rates of motion than Ni2+-inhibited cells. Short segments from the distal tip of the flagellum contain only the 9 + 2 microtubular axoneme. These short segments are able to propagate imposed bends even in the presence of Ni2+. In addition to wave propagation Ni2+-treated sperm can be shown to exhibit a normal sliding tubule phenomenon by direct assay. Although Ni2+-treated cells have a functional sliding tubule mechanism, and consequently the axoneme can propagate bends, it appears that these retained functions are not sufficient to cause spontaneous bend initiation. Our findings show that bend initiation is inhibited by Ni2+, and therefore is an independent process separate from the sliding tubule mechanism responsible for wave propagation.
Authors:
C B Lindemann; I Fentie; R Rikmenspoel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  87     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-01-26     Completed Date:  1981-01-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  420-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Membrane / physiology
Depression, Chemical
Dithiothreitol / pharmacology
Humans
Male
Microtubules / physiology
Nickel / pharmacology*
Sperm Motility / drug effects*
Sperm Tail / drug effects*
Spermatozoa / drug effects*
Grant Support
ID/Acronym/Agency:
HD-08752/HD/NICHD NIH HHS; HD-12535/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
3483-12-3/Dithiothreitol; 7440-02-0/Nickel
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1980
Volume: 87 Issue: 2
First Page: 420 Last Page: 426
ID: 2110741
Publisher Id: 81047086
PubMed Id: 7430248

A selective effect of Ni2+ on wave initiation in bull sperm flagella


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