| The selection of Chinese hamster cells deficient in oxidative energy metabolism. | |
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MedLine Citation:
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PMID: 1027147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A selection scheme based on the nutritional requirements of a previously described respiration-deficient Chinese hamster line has been used to isolate new mutants defective in oxidative energy metabolism. Three of the primary characteristics of this type of mutant are (1) a strict dependency on the continued presence of glucose for survival; (2) a drastic reduction in the rate of oxygen consumption; (3) an inhibition of Krebs cycle activity resulting in auxotrophy for asparagine and carbon dioxide. In the case of one cell line which was used (V79), up to 65% of the survivors of a selection were found to possess this phenotype after only one round of selection. By contrast, it proved much more difficult to obtain such mutants from another cell line (CCL16). A preliminary characterization of a number of these mutants is presented. |
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Authors:
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G Ditta; K Soderberg; F Landy; I E Scheffler |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Somatic cell genetics Volume: 2 ISSN: 0098-0366 ISO Abbreviation: Somatic Cell Genet. Publication Date: 1976 Jul |
Date Detail:
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Created Date: 1977-07-29 Completed Date: 1977-07-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7506054 Medline TA: Somatic Cell Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 331-44 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asparagine / metabolism Carbon Dioxide / metabolism Cell Line Cell Survival Cells, Cultured Citric Acid Cycle Cricetinae Energy Metabolism* Glucose / metabolism Mutation* Oxygen Consumption Phenotype Selection, Genetic |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide; 50-99-7/Glucose; 7006-34-0/Asparagine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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