Document Detail

The selection of Chinese hamster cells deficient in oxidative energy metabolism.
MedLine Citation:
PMID:  1027147     Owner:  NLM     Status:  MEDLINE    
A selection scheme based on the nutritional requirements of a previously described respiration-deficient Chinese hamster line has been used to isolate new mutants defective in oxidative energy metabolism. Three of the primary characteristics of this type of mutant are (1) a strict dependency on the continued presence of glucose for survival; (2) a drastic reduction in the rate of oxygen consumption; (3) an inhibition of Krebs cycle activity resulting in auxotrophy for asparagine and carbon dioxide. In the case of one cell line which was used (V79), up to 65% of the survivors of a selection were found to possess this phenotype after only one round of selection. By contrast, it proved much more difficult to obtain such mutants from another cell line (CCL16). A preliminary characterization of a number of these mutants is presented.
G Ditta; K Soderberg; F Landy; I E Scheffler
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  2     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1977-07-29     Completed Date:  1977-07-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  331-44     Citation Subset:  IM    
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MeSH Terms
Asparagine / metabolism
Carbon Dioxide / metabolism
Cell Line
Cell Survival
Cells, Cultured
Citric Acid Cycle
Energy Metabolism*
Glucose / metabolism
Oxygen Consumption
Selection, Genetic
Reg. No./Substance:
124-38-9/Carbon Dioxide; 50-99-7/Glucose; 7006-34-0/Asparagine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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