Document Detail


The selection of Chinese hamster cells deficient in oxidative energy metabolism.
MedLine Citation:
PMID:  1027147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A selection scheme based on the nutritional requirements of a previously described respiration-deficient Chinese hamster line has been used to isolate new mutants defective in oxidative energy metabolism. Three of the primary characteristics of this type of mutant are (1) a strict dependency on the continued presence of glucose for survival; (2) a drastic reduction in the rate of oxygen consumption; (3) an inhibition of Krebs cycle activity resulting in auxotrophy for asparagine and carbon dioxide. In the case of one cell line which was used (V79), up to 65% of the survivors of a selection were found to possess this phenotype after only one round of selection. By contrast, it proved much more difficult to obtain such mutants from another cell line (CCL16). A preliminary characterization of a number of these mutants is presented.
Authors:
G Ditta; K Soderberg; F Landy; I E Scheffler
Related Documents :
3545447 - Rat hepatocyte-mediated bacterial mutagenicity in relation to the carcinogenic potency ...
2450277 - Bleomycin and x-ray-hypersensitive chinese hamster ovary cell mutants: genetic analysis...
16527357 - Genotoxicity and transmission electron microscopy studies of molybdocene dichloride.
1995947 - Defective transport of sindbis virus glycoproteins in end4 mutant chinese hamster ovary...
21757357 - Two-step bioorthogonal activity-based proteasome profiling using copper-free click reag...
11493977 - The incidence of apoptosis during colorectal tumorigenesis.
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  2     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1977-07-29     Completed Date:  1977-07-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  331-44     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Asparagine / metabolism
Carbon Dioxide / metabolism
Cell Line
Cell Survival
Cells, Cultured
Citric Acid Cycle
Cricetinae
Energy Metabolism*
Glucose / metabolism
Mutation*
Oxygen Consumption
Phenotype
Selection, Genetic
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 50-99-7/Glucose; 7006-34-0/Asparagine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effect of specific antiserum on the metabolism of three membrane-associated antigens of ASL-1 x ...
Next Document:  Transfer of the human X chromosome to human--Chinese hamster cell hybrids via isolated HeLa metaphas...