Document Detail


The seed stock concept and quality control for cell lines.
MedLine Citation:
PMID:  3044184     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Procedures for the characterization of cell culture lines are reviewed. It is recommended that seed stocks be developed and that major quality-control efforts be applied first to those holdings, with essential tests repeated on cultures from working or distribution stocks prepared subsequently. Steps are taken throughout to ensure absence of microbial contaminants. Mycoplasma are especially troublesome. Investigators need also to be aware of potential endogenous viruses which may be hazardous or interfere with interpretation of results. Cell line species and absolute identities are established by fluorescent antibody staining, by determination of isoenzyme profiles, and by cytogenetic analyses. Additional characterizations will vary with cell type but may include tests for specific products, cytoskeletal proteins, tissue specific antigens, or immunoglobulins. Credentials of lines added to a collection plus representative cultures can be returned to the originators for concurrence before distribution. Advisory committees may be established as circumstance dictates to provide additional expertise regarding quality control for the specific collection being developed.
Authors:
R J Hay
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Analytical biochemistry     Volume:  171     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-09-16     Completed Date:  1988-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  225-37     Citation Subset:  IM    
Affiliation:
Cell Culture Department, American Type Culture Collection, Rockville, Maryland 20852.
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MeSH Terms
Descriptor/Qualifier:
Cell Line*
Cytological Techniques
Quality Control
Grant Support
ID/Acronym/Agency:
N01-CB-71014/CB/NCI NIH HHS; N01-RR-2-2117/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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